Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization

Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization

The genome is folded in 3-dimensions, though the lack of robust ultra-resolution imaging makes this difficult to visualise. Here, the authors present 3D-EMISH that combines serial block-face scanning electron microscopy with in situ hybridization.

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Detalles Bibliográficos
Autores principales: Paweł Trzaskoma, Błażej Ruszczycki, Byoungkoo Lee, Katarzyna K. Pels, Katarzyna Krawczyk, Grzegorz Bokota, Andrzej A. Szczepankiewicz, Jesse Aaron, Agnieszka Walczak, Małgorzata A. Śliwińska, Adriana Magalska, Michal Kadlof, Artur Wolny, Zofia Parteka, Sebastian Arabasz, Magdalena Kiss-Arabasz, Dariusz Plewczyński, Yijun Ruan, Grzegorz M. Wilczyński
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
Materias:
Science
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Acceso en línea:https://doaj.org/article/314efe399d9e425fbc4fd722009456ec
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Sumario:The genome is folded in 3-dimensions, though the lack of robust ultra-resolution imaging makes this difficult to visualise. Here, the authors present 3D-EMISH that combines serial block-face scanning electron microscopy with in situ hybridization.

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