Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization
The genome is folded in 3-dimensions, though the lack of robust ultra-resolution imaging makes this difficult to visualise. Here, the authors present 3D-EMISH that combines serial block-face scanning electron microscopy with in situ hybridization.
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/314efe399d9e425fbc4fd722009456ec |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:314efe399d9e425fbc4fd722009456ec |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:314efe399d9e425fbc4fd722009456ec2021-12-02T17:32:27ZUltrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization10.1038/s41467-020-15987-22041-1723https://doaj.org/article/314efe399d9e425fbc4fd722009456ec2020-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15987-2https://doaj.org/toc/2041-1723The genome is folded in 3-dimensions, though the lack of robust ultra-resolution imaging makes this difficult to visualise. Here, the authors present 3D-EMISH that combines serial block-face scanning electron microscopy with in situ hybridization.Paweł TrzaskomaBłażej RuszczyckiByoungkoo LeeKatarzyna K. PelsKatarzyna KrawczykGrzegorz BokotaAndrzej A. SzczepankiewiczJesse AaronAgnieszka WalczakMałgorzata A. ŚliwińskaAdriana MagalskaMichal KadlofArtur WolnyZofia PartekaSebastian ArabaszMagdalena Kiss-ArabaszDariusz PlewczyńskiYijun RuanGrzegorz M. WilczyńskiNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Paweł Trzaskoma Błażej Ruszczycki Byoungkoo Lee Katarzyna K. Pels Katarzyna Krawczyk Grzegorz Bokota Andrzej A. Szczepankiewicz Jesse Aaron Agnieszka Walczak Małgorzata A. Śliwińska Adriana Magalska Michal Kadlof Artur Wolny Zofia Parteka Sebastian Arabasz Magdalena Kiss-Arabasz Dariusz Plewczyński Yijun Ruan Grzegorz M. Wilczyński Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization |
| description |
The genome is folded in 3-dimensions, though the lack of robust ultra-resolution imaging makes this difficult to visualise. Here, the authors present 3D-EMISH that combines serial block-face scanning electron microscopy with in situ hybridization. |
| format |
article |
| author |
Paweł Trzaskoma Błażej Ruszczycki Byoungkoo Lee Katarzyna K. Pels Katarzyna Krawczyk Grzegorz Bokota Andrzej A. Szczepankiewicz Jesse Aaron Agnieszka Walczak Małgorzata A. Śliwińska Adriana Magalska Michal Kadlof Artur Wolny Zofia Parteka Sebastian Arabasz Magdalena Kiss-Arabasz Dariusz Plewczyński Yijun Ruan Grzegorz M. Wilczyński |
| author_facet |
Paweł Trzaskoma Błażej Ruszczycki Byoungkoo Lee Katarzyna K. Pels Katarzyna Krawczyk Grzegorz Bokota Andrzej A. Szczepankiewicz Jesse Aaron Agnieszka Walczak Małgorzata A. Śliwińska Adriana Magalska Michal Kadlof Artur Wolny Zofia Parteka Sebastian Arabasz Magdalena Kiss-Arabasz Dariusz Plewczyński Yijun Ruan Grzegorz M. Wilczyński |
| author_sort |
Paweł Trzaskoma |
| title |
Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization |
| title_short |
Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization |
| title_full |
Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization |
| title_fullStr |
Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization |
| title_full_unstemmed |
Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization |
| title_sort |
ultrastructural visualization of 3d chromatin folding using volume electron microscopy and dna in situ hybridization |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/314efe399d9e425fbc4fd722009456ec |
| work_keys_str_mv |
AT pawełtrzaskoma ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT błazejruszczycki ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT byoungkoolee ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT katarzynakpels ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT katarzynakrawczyk ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT grzegorzbokota ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT andrzejaszczepankiewicz ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT jesseaaron ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT agnieszkawalczak ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT małgorzataasliwinska ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT adrianamagalska ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT michalkadlof ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT arturwolny ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT zofiaparteka ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT sebastianarabasz ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT magdalenakissarabasz ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT dariuszplewczynski ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT yijunruan ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization AT grzegorzmwilczynski ultrastructuralvisualizationof3dchromatinfoldingusingvolumeelectronmicroscopyanddnainsituhybridization |
| _version_ |
1718380299032723456 |