Protective Effects of Melatonin on <i>Saccharomyces cerevisiae</i> under Ethanol Stress

During alcoholic fermentation, <i>Saccharomyces cerevisiae</i> is subjected to several stresses, among which ethanol is of capital importance. Melatonin, a bioactive molecule synthesized by yeast during alcoholic fermentation, has an antioxidant role and is proposed to contribute to coun...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mercè Sunyer-Figueres, Albert Mas, Gemma Beltran, María-Jesús Torija
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ethanol stress yeast antioxidant response melatonin supplementation ROS accumulation catalase activity superoxide dismutase Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/338fdde74a9049a8bf60ace50d35e8d4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:338fdde74a9049a8bf60ace50d35e8d4
record_format	dspace
spelling	oai:doaj.org-article:338fdde74a9049a8bf60ace50d35e8d42021-11-25T16:27:28ZProtective Effects of Melatonin on <i>Saccharomyces cerevisiae</i> under Ethanol Stress10.3390/antiox101117352076-3921https://doaj.org/article/338fdde74a9049a8bf60ace50d35e8d42021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1735https://doaj.org/toc/2076-3921During alcoholic fermentation, <i>Saccharomyces cerevisiae</i> is subjected to several stresses, among which ethanol is of capital importance. Melatonin, a bioactive molecule synthesized by yeast during alcoholic fermentation, has an antioxidant role and is proposed to contribute to counteracting fermentation-associated stresses. The aim of this study was to unravel the protective effect of melatonin on yeast cells subjected to ethanol stress. For that purpose, the effect of ethanol concentrations (6 to 12%) on a wine strain and a lab strain of <i>S. cerevisiae</i> was evaluated, monitoring the viability, growth capacity, mortality, and several indicators of oxidative stress over time, such as reactive oxygen species (ROS) accumulation, lipid peroxidation, and the activity of catalase and superoxide dismutase enzymes. In general, ethanol exposure reduced the cell growth of <i>S. cerevisiae</i> and increased mortality, ROS accumulation, lipid peroxidation and antioxidant enzyme activity. Melatonin supplementation softened the effect of ethanol, enhancing cell growth and decreasing oxidative damage by lowering ROS accumulation, lipid peroxidation, and antioxidant enzyme activities. However, the effects of melatonin were dependent on strain, melatonin concentration, and growth phase. The results of this study indicate that melatonin has a protective role against mild ethanol stress, mainly by reducing the oxidative stress triggered by this alcohol.Mercè Sunyer-FigueresAlbert MasGemma BeltranMaría-Jesús TorijaMDPI AGarticleethanol stressyeast antioxidant responsemelatonin supplementationROS accumulationcatalase activitysuperoxide dismutaseTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1735, p 1735 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ethanol stress yeast antioxidant response melatonin supplementation ROS accumulation catalase activity superoxide dismutase Therapeutics. Pharmacology RM1-950
spellingShingle	ethanol stress yeast antioxidant response melatonin supplementation ROS accumulation catalase activity superoxide dismutase Therapeutics. Pharmacology RM1-950 Mercè Sunyer-Figueres Albert Mas Gemma Beltran María-Jesús Torija Protective Effects of Melatonin on <i>Saccharomyces cerevisiae</i> under Ethanol Stress
description	During alcoholic fermentation, <i>Saccharomyces cerevisiae</i> is subjected to several stresses, among which ethanol is of capital importance. Melatonin, a bioactive molecule synthesized by yeast during alcoholic fermentation, has an antioxidant role and is proposed to contribute to counteracting fermentation-associated stresses. The aim of this study was to unravel the protective effect of melatonin on yeast cells subjected to ethanol stress. For that purpose, the effect of ethanol concentrations (6 to 12%) on a wine strain and a lab strain of <i>S. cerevisiae</i> was evaluated, monitoring the viability, growth capacity, mortality, and several indicators of oxidative stress over time, such as reactive oxygen species (ROS) accumulation, lipid peroxidation, and the activity of catalase and superoxide dismutase enzymes. In general, ethanol exposure reduced the cell growth of <i>S. cerevisiae</i> and increased mortality, ROS accumulation, lipid peroxidation and antioxidant enzyme activity. Melatonin supplementation softened the effect of ethanol, enhancing cell growth and decreasing oxidative damage by lowering ROS accumulation, lipid peroxidation, and antioxidant enzyme activities. However, the effects of melatonin were dependent on strain, melatonin concentration, and growth phase. The results of this study indicate that melatonin has a protective role against mild ethanol stress, mainly by reducing the oxidative stress triggered by this alcohol.
format	article
author	Mercè Sunyer-Figueres Albert Mas Gemma Beltran María-Jesús Torija
author_facet	Mercè Sunyer-Figueres Albert Mas Gemma Beltran María-Jesús Torija
author_sort	Mercè Sunyer-Figueres
title	Protective Effects of Melatonin on <i>Saccharomyces cerevisiae</i> under Ethanol Stress
title_short	Protective Effects of Melatonin on <i>Saccharomyces cerevisiae</i> under Ethanol Stress
title_full	Protective Effects of Melatonin on <i>Saccharomyces cerevisiae</i> under Ethanol Stress
title_fullStr	Protective Effects of Melatonin on <i>Saccharomyces cerevisiae</i> under Ethanol Stress
title_full_unstemmed	Protective Effects of Melatonin on <i>Saccharomyces cerevisiae</i> under Ethanol Stress
title_sort	protective effects of melatonin on <i>saccharomyces cerevisiae</i> under ethanol stress
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/338fdde74a9049a8bf60ace50d35e8d4
work_keys_str_mv	AT mercesunyerfigueres protectiveeffectsofmelatoninonisaccharomycescerevisiaeiunderethanolstress AT albertmas protectiveeffectsofmelatoninonisaccharomycescerevisiaeiunderethanolstress AT gemmabeltran protectiveeffectsofmelatoninonisaccharomycescerevisiaeiunderethanolstress AT mariajesustorija protectiveeffectsofmelatoninonisaccharomycescerevisiaeiunderethanolstress
_version_	1718413128258027520

Protective Effects of Melatonin on <i>Saccharomyces cerevisiae</i> under Ethanol Stress

Ejemplares similares