Phenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives

Abstract. Akroman R, Nurhayati N, Suwasono S, Jayus J. 2019. Phenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives. Biodiversitas 20: 2468-2474. As the demand for materials suitable for edible film in food processing is increasing, the exploratio...

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Autores principales:	Robby Akroman, NURHAYATI NURHAYATI, SONY SUWASONO, JAY JAYUS
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	culvularia lunata, exopolysaccharide, genotypic, phenotypic Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/51f3175f6eee4fe7bfc45ec00b418b86
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spelling	oai:doaj.org-article:51f3175f6eee4fe7bfc45ec00b418b862021-11-21T21:54:26ZPhenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives1412-033X2085-472210.13057/biodiv/d200906https://doaj.org/article/51f3175f6eee4fe7bfc45ec00b418b862019-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3895https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Akroman R, Nurhayati N, Suwasono S, Jayus J. 2019. Phenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives. Biodiversitas 20: 2468-2474. As the demand for materials suitable for edible film in food processing is increasing, the exploration of exopolysaccharide (EPS) producing fungi is important. Some fungi had been reported to produce extracellular polysaccharide having rheological properties potential as thickener and or plasticizer useful for edible film raw material. Therefore, this study was focused on isolation and identification of EPS-producing fungi from the plantation area surrounding the University of Jember, Indonesia. The fungal isolate obtained was morphologically identified based on mycelia and spore type, while its genotype was determined using ITS1 and ITS4 primers. The optimum initial culture pH for EPS production was measured using shake flask culture fermentation. The rheological properties of the EPS were determined using Brookfield viscometer, and surface structure of EPS was observed using a scanning electron microscope (SEM). The result showed that the mycelium of the isolate was reddish-brown and septated, its spores were formed as conidiospores. Internal Transcribed Spacer (ITS) sequence of the fungus showed that the isolate is a new species. Therefore, it was designated as C. lunata isolate RJ01. Optimum initial pH for EPS production was pH 6.0, achieved after 5 days fermentation yielded 2.6 g L-1 of EPS. The EPS from C. lunata isolate RJ01 have no cytotoxic activity on vero cell. The rheological behaviour of C. lunata EPS was viscous, and its surface structure of EPS was compact indicating its potency as thickening agent and plasticizer, a material required for edible film formation in food industries.Robby AkromanNURHAYATI NURHAYATISONY SUWASONOJAY JAYUSMBI & UNS Soloarticleculvularia lunata, exopolysaccharide, genotypic, phenotypicBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	culvularia lunata, exopolysaccharide, genotypic, phenotypic Biology (General) QH301-705.5
spellingShingle	culvularia lunata, exopolysaccharide, genotypic, phenotypic Biology (General) QH301-705.5 Robby Akroman NURHAYATI NURHAYATI SONY SUWASONO JAY JAYUS Phenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives
description	Abstract. Akroman R, Nurhayati N, Suwasono S, Jayus J. 2019. Phenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives. Biodiversitas 20: 2468-2474. As the demand for materials suitable for edible film in food processing is increasing, the exploration of exopolysaccharide (EPS) producing fungi is important. Some fungi had been reported to produce extracellular polysaccharide having rheological properties potential as thickener and or plasticizer useful for edible film raw material. Therefore, this study was focused on isolation and identification of EPS-producing fungi from the plantation area surrounding the University of Jember, Indonesia. The fungal isolate obtained was morphologically identified based on mycelia and spore type, while its genotype was determined using ITS1 and ITS4 primers. The optimum initial culture pH for EPS production was measured using shake flask culture fermentation. The rheological properties of the EPS were determined using Brookfield viscometer, and surface structure of EPS was observed using a scanning electron microscope (SEM). The result showed that the mycelium of the isolate was reddish-brown and septated, its spores were formed as conidiospores. Internal Transcribed Spacer (ITS) sequence of the fungus showed that the isolate is a new species. Therefore, it was designated as C. lunata isolate RJ01. Optimum initial pH for EPS production was pH 6.0, achieved after 5 days fermentation yielded 2.6 g L-1 of EPS. The EPS from C. lunata isolate RJ01 have no cytotoxic activity on vero cell. The rheological behaviour of C. lunata EPS was viscous, and its surface structure of EPS was compact indicating its potency as thickening agent and plasticizer, a material required for edible film formation in food industries.
format	article
author	Robby Akroman NURHAYATI NURHAYATI SONY SUWASONO JAY JAYUS
author_facet	Robby Akroman NURHAYATI NURHAYATI SONY SUWASONO JAY JAYUS
author_sort	Robby Akroman
title	Phenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives
title_short	Phenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives
title_full	Phenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives
title_fullStr	Phenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives
title_full_unstemmed	Phenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives
title_sort	phenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/51f3175f6eee4fe7bfc45ec00b418b86
work_keys_str_mv	AT robbyakroman phenotypicandgenotypiccharacteristicsofexopolysaccharideproducingfungiasasourceoffoodadditives AT nurhayatinurhayati phenotypicandgenotypiccharacteristicsofexopolysaccharideproducingfungiasasourceoffoodadditives AT sonysuwasono phenotypicandgenotypiccharacteristicsofexopolysaccharideproducingfungiasasourceoffoodadditives AT jayjayus phenotypicandgenotypiccharacteristicsofexopolysaccharideproducingfungiasasourceoffoodadditives
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Phenotypic and genotypic characteristics of exopolysaccharide-producing fungi as a source of food additives

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