A thermostable Cas9 with increased lifetime in human plasma

While current CRISPR-Cas9 tools have revolutionized genome editing, they are not suitable for applications at elevated temperatures. Here, the authors characterize GeoCas9 from Geobacillus stearothermophilus, which is active up to 70°C and is stable in human plasma.

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Bibliographic Details
Main Authors: Lucas B. Harrington, David Paez-Espino, Brett T. Staahl, Janice S. Chen, Enbo Ma, Nikos C. Kyrpides, Jennifer A. Doudna
Format: article
Language:EN
Published: Nature Portfolio 2017
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Online Access:https://doaj.org/article/5515b76d1431477696220e607e0ddca5
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