A thermostable Cas9 with increased lifetime in human plasma

While current CRISPR-Cas9 tools have revolutionized genome editing, they are not suitable for applications at elevated temperatures. Here, the authors characterize GeoCas9 from Geobacillus stearothermophilus, which is active up to 70°C and is stable in human plasma.

Guardado en:

Detalles Bibliográficos
Autores principales:	Lucas B. Harrington, David Paez-Espino, Brett T. Staahl, Janice S. Chen, Enbo Ma, Nikos C. Kyrpides, Jennifer A. Doudna
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/5515b76d1431477696220e607e0ddca5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5515b76d1431477696220e607e0ddca5
record_format	dspace
spelling	oai:doaj.org-article:5515b76d1431477696220e607e0ddca52021-12-02T14:41:59ZA thermostable Cas9 with increased lifetime in human plasma10.1038/s41467-017-01408-42041-1723https://doaj.org/article/5515b76d1431477696220e607e0ddca52017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01408-4https://doaj.org/toc/2041-1723While current CRISPR-Cas9 tools have revolutionized genome editing, they are not suitable for applications at elevated temperatures. Here, the authors characterize GeoCas9 from Geobacillus stearothermophilus, which is active up to 70°C and is stable in human plasma.Lucas B. HarringtonDavid Paez-EspinoBrett T. StaahlJanice S. ChenEnbo MaNikos C. KyrpidesJennifer A. DoudnaNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Lucas B. Harrington David Paez-Espino Brett T. Staahl Janice S. Chen Enbo Ma Nikos C. Kyrpides Jennifer A. Doudna A thermostable Cas9 with increased lifetime in human plasma
description	While current CRISPR-Cas9 tools have revolutionized genome editing, they are not suitable for applications at elevated temperatures. Here, the authors characterize GeoCas9 from Geobacillus stearothermophilus, which is active up to 70°C and is stable in human plasma.
format	article
author	Lucas B. Harrington David Paez-Espino Brett T. Staahl Janice S. Chen Enbo Ma Nikos C. Kyrpides Jennifer A. Doudna
author_facet	Lucas B. Harrington David Paez-Espino Brett T. Staahl Janice S. Chen Enbo Ma Nikos C. Kyrpides Jennifer A. Doudna
author_sort	Lucas B. Harrington
title	A thermostable Cas9 with increased lifetime in human plasma
title_short	A thermostable Cas9 with increased lifetime in human plasma
title_full	A thermostable Cas9 with increased lifetime in human plasma
title_fullStr	A thermostable Cas9 with increased lifetime in human plasma
title_full_unstemmed	A thermostable Cas9 with increased lifetime in human plasma
title_sort	thermostable cas9 with increased lifetime in human plasma
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5515b76d1431477696220e607e0ddca5
work_keys_str_mv	AT lucasbharrington athermostablecas9withincreasedlifetimeinhumanplasma AT davidpaezespino athermostablecas9withincreasedlifetimeinhumanplasma AT bretttstaahl athermostablecas9withincreasedlifetimeinhumanplasma AT janiceschen athermostablecas9withincreasedlifetimeinhumanplasma AT enboma athermostablecas9withincreasedlifetimeinhumanplasma AT nikosckyrpides athermostablecas9withincreasedlifetimeinhumanplasma AT jenniferadoudna athermostablecas9withincreasedlifetimeinhumanplasma AT lucasbharrington thermostablecas9withincreasedlifetimeinhumanplasma AT davidpaezespino thermostablecas9withincreasedlifetimeinhumanplasma AT bretttstaahl thermostablecas9withincreasedlifetimeinhumanplasma AT janiceschen thermostablecas9withincreasedlifetimeinhumanplasma AT enboma thermostablecas9withincreasedlifetimeinhumanplasma AT nikosckyrpides thermostablecas9withincreasedlifetimeinhumanplasma AT jenniferadoudna thermostablecas9withincreasedlifetimeinhumanplasma
_version_	1718389808255991808

A thermostable Cas9 with increased lifetime in human plasma

Ejemplares similares