In vitro Cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives

Several different genetic strategies have been reported for the modification of polyketide synthases but the highly repetitive modular structure makes this difficult. Here the authors report on an adapted Cas9 reaction and Gibson assembly to edit a target region of the polyketide synthases gene in v...

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Autores principales:	Kei Kudo, Takuya Hashimoto, Junko Hashimoto, Ikuko Kozone, Noritaka Kagaya, Reiko Ueoka, Takehiro Nishimura, Mamoru Komatsu, Hikaru Suenaga, Haruo Ikeda, Kazuo Shin-ya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6d001e0fbfd74c5b9143e6e06438d202
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spelling	oai:doaj.org-article:6d001e0fbfd74c5b9143e6e06438d2022021-12-02T15:07:56ZIn vitro Cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives10.1038/s41467-020-17769-22041-1723https://doaj.org/article/6d001e0fbfd74c5b9143e6e06438d2022020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17769-2https://doaj.org/toc/2041-1723Several different genetic strategies have been reported for the modification of polyketide synthases but the highly repetitive modular structure makes this difficult. Here the authors report on an adapted Cas9 reaction and Gibson assembly to edit a target region of the polyketide synthases gene in vitro.Kei KudoTakuya HashimotoJunko HashimotoIkuko KozoneNoritaka KagayaReiko UeokaTakehiro NishimuraMamoru KomatsuHikaru SuenagaHaruo IkedaKazuo Shin-yaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Kei Kudo Takuya Hashimoto Junko Hashimoto Ikuko Kozone Noritaka Kagaya Reiko Ueoka Takehiro Nishimura Mamoru Komatsu Hikaru Suenaga Haruo Ikeda Kazuo Shin-ya In vitro Cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives
description	Several different genetic strategies have been reported for the modification of polyketide synthases but the highly repetitive modular structure makes this difficult. Here the authors report on an adapted Cas9 reaction and Gibson assembly to edit a target region of the polyketide synthases gene in vitro.
format	article
author	Kei Kudo Takuya Hashimoto Junko Hashimoto Ikuko Kozone Noritaka Kagaya Reiko Ueoka Takehiro Nishimura Mamoru Komatsu Hikaru Suenaga Haruo Ikeda Kazuo Shin-ya
author_facet	Kei Kudo Takuya Hashimoto Junko Hashimoto Ikuko Kozone Noritaka Kagaya Reiko Ueoka Takehiro Nishimura Mamoru Komatsu Hikaru Suenaga Haruo Ikeda Kazuo Shin-ya
author_sort	Kei Kudo
title	In vitro Cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives
title_short	In vitro Cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives
title_full	In vitro Cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives
title_fullStr	In vitro Cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives
title_full_unstemmed	In vitro Cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives
title_sort	in vitro cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/6d001e0fbfd74c5b9143e6e06438d202
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In vitro Cas9-assisted editing of modular polyketide synthase genes to produce desired natural product derivatives

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