Inhibitory activity of crude bacteriocin produced by lactic acid bacteria isolated from dadih against Listeria monocytogenes

The use of natural preservatives called bacteriocin derived from lactic acid bacteria (LAB) is one way of preventing food from being contaminated by pathogenic microorganisms such as L. monocytogenes (LM). The aims of this study were to evaluate the ability of LAB isolated from dadih to inhibit the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Usman Pato, YUSMARINI YUSUF, SHANTI FITRIANI, NIA NAIDYA JONNADI, MIMI SRI WAHYUNI, JESKA ARYANINGSIH FERUNI, IRWANDI JASWIR
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	antimicrobial activity, lactic acid bacteria, listeria monocytogenes, bacteriocin, preservative compound Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/70516b3dea434010844578de70316974
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:70516b3dea434010844578de70316974
record_format	dspace
spelling	oai:doaj.org-article:70516b3dea434010844578de703169742021-11-22T00:34:40ZInhibitory activity of crude bacteriocin produced by lactic acid bacteria isolated from dadih against Listeria monocytogenes1412-033X2085-472210.13057/biodiv/d210404https://doaj.org/article/70516b3dea434010844578de703169742020-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5143https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722The use of natural preservatives called bacteriocin derived from lactic acid bacteria (LAB) is one way of preventing food from being contaminated by pathogenic microorganisms such as L. monocytogenes (LM). The aims of this study were to evaluate the ability of LAB isolated from dadih to inhibit the growth of LM and to obtain the antimicrobial components that play a role in inhibiting the growth of LM. The antimicrobial activity of the supernatant obtained from 12 strains of dadih LAB was determined using the paper disk diffusion method. The results showed that the supernatant from the 12 LAB strains was able to inhibit the growth of LM with various inhibition zones. However, out of the 12 LABs, only 9 strains were found to have an inhibition zone of more than 3.5 mm. The antimicrobial compounds of 9 strains were tested and it was found that the antimicrobial compounds of strains R-8, R-14 and R-49 were derived from lactic acid. In addition, 6 strains namely R-43, R-32, R-19, R-55, R-45 and R-41 were derived from bacteriocin based on their sensitivity to pH, heat and enzyme treatments. Crude bacteriocin derived from 6 LAB strains inhibited the growth of LM, and the highest antimicrobial activity was obtained in Streptococcus faecalis subsp. liquefaciens R-55 with an average inhibition zone of 13.87 mm. Bacteriocin produced by strain R-55 can be used as natural preservatives for the prevention of food-borne disease caused by LM.Usman PatoYUSMARINI YUSUFSHANTI FITRIANINIA NAIDYA JONNADIMIMI SRI WAHYUNIJESKA ARYANINGSIH FERUNIIRWANDI JASWIRMBI & UNS Soloarticleantimicrobial activity, lactic acid bacteria, listeria monocytogenes, bacteriocin, preservative compoundBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial activity, lactic acid bacteria, listeria monocytogenes, bacteriocin, preservative compound Biology (General) QH301-705.5
spellingShingle	antimicrobial activity, lactic acid bacteria, listeria monocytogenes, bacteriocin, preservative compound Biology (General) QH301-705.5 Usman Pato YUSMARINI YUSUF SHANTI FITRIANI NIA NAIDYA JONNADI MIMI SRI WAHYUNI JESKA ARYANINGSIH FERUNI IRWANDI JASWIR Inhibitory activity of crude bacteriocin produced by lactic acid bacteria isolated from dadih against Listeria monocytogenes
description	The use of natural preservatives called bacteriocin derived from lactic acid bacteria (LAB) is one way of preventing food from being contaminated by pathogenic microorganisms such as L. monocytogenes (LM). The aims of this study were to evaluate the ability of LAB isolated from dadih to inhibit the growth of LM and to obtain the antimicrobial components that play a role in inhibiting the growth of LM. The antimicrobial activity of the supernatant obtained from 12 strains of dadih LAB was determined using the paper disk diffusion method. The results showed that the supernatant from the 12 LAB strains was able to inhibit the growth of LM with various inhibition zones. However, out of the 12 LABs, only 9 strains were found to have an inhibition zone of more than 3.5 mm. The antimicrobial compounds of 9 strains were tested and it was found that the antimicrobial compounds of strains R-8, R-14 and R-49 were derived from lactic acid. In addition, 6 strains namely R-43, R-32, R-19, R-55, R-45 and R-41 were derived from bacteriocin based on their sensitivity to pH, heat and enzyme treatments. Crude bacteriocin derived from 6 LAB strains inhibited the growth of LM, and the highest antimicrobial activity was obtained in Streptococcus faecalis subsp. liquefaciens R-55 with an average inhibition zone of 13.87 mm. Bacteriocin produced by strain R-55 can be used as natural preservatives for the prevention of food-borne disease caused by LM.
format	article
author	Usman Pato YUSMARINI YUSUF SHANTI FITRIANI NIA NAIDYA JONNADI MIMI SRI WAHYUNI JESKA ARYANINGSIH FERUNI IRWANDI JASWIR
author_facet	Usman Pato YUSMARINI YUSUF SHANTI FITRIANI NIA NAIDYA JONNADI MIMI SRI WAHYUNI JESKA ARYANINGSIH FERUNI IRWANDI JASWIR
author_sort	Usman Pato
title	Inhibitory activity of crude bacteriocin produced by lactic acid bacteria isolated from dadih against Listeria monocytogenes
title_short	Inhibitory activity of crude bacteriocin produced by lactic acid bacteria isolated from dadih against Listeria monocytogenes
title_full	Inhibitory activity of crude bacteriocin produced by lactic acid bacteria isolated from dadih against Listeria monocytogenes
title_fullStr	Inhibitory activity of crude bacteriocin produced by lactic acid bacteria isolated from dadih against Listeria monocytogenes
title_full_unstemmed	Inhibitory activity of crude bacteriocin produced by lactic acid bacteria isolated from dadih against Listeria monocytogenes
title_sort	inhibitory activity of crude bacteriocin produced by lactic acid bacteria isolated from dadih against listeria monocytogenes
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/70516b3dea434010844578de70316974
work_keys_str_mv	AT usmanpato inhibitoryactivityofcrudebacteriocinproducedbylacticacidbacteriaisolatedfromdadihagainstlisteriamonocytogenes AT yusmariniyusuf inhibitoryactivityofcrudebacteriocinproducedbylacticacidbacteriaisolatedfromdadihagainstlisteriamonocytogenes AT shantifitriani inhibitoryactivityofcrudebacteriocinproducedbylacticacidbacteriaisolatedfromdadihagainstlisteriamonocytogenes AT nianaidyajonnadi inhibitoryactivityofcrudebacteriocinproducedbylacticacidbacteriaisolatedfromdadihagainstlisteriamonocytogenes AT mimisriwahyuni inhibitoryactivityofcrudebacteriocinproducedbylacticacidbacteriaisolatedfromdadihagainstlisteriamonocytogenes AT jeskaaryaningsihferuni inhibitoryactivityofcrudebacteriocinproducedbylacticacidbacteriaisolatedfromdadihagainstlisteriamonocytogenes AT irwandijaswir inhibitoryactivityofcrudebacteriocinproducedbylacticacidbacteriaisolatedfromdadihagainstlisteriamonocytogenes
_version_	1718418588031778816

Inhibitory activity of crude bacteriocin produced by lactic acid bacteria isolated from dadih against Listeria monocytogenes

Ejemplares similares