Antibacterial susceptability of E. coli strains isolated from raw milk

Introduction. The processing of most raw milk products can lead to contamination with unwanted microflora due to poor sanitation and hygienic conditions. The inadequate antibiotic use over the past decades has led to the emergence and wide spread of bacterial populations, particularly of Escherichia...

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Autores principales:	Valerii USHKALOV, Vyacheslav DANCHUK, Artem USHKALOV, Aidyn SALMANOV, Yuriy VISHOVAN, Sergiy BOIANOVSKIY, Sergiy TERESHCHENKO, Liliana DAVYDOVSKA
Formato:	article
Lenguaje:	EN FR RO RU
Publicado:	Asociatia de Biosiguranta si Biosecuritate 2021
Materias:	escherichia coli strains microorganisms raw milk antibiotic resistance susceptibility Medicine R Science Q
Acceso en línea:	https://doaj.org/article/91895aacf521486384904a7e5e5bb7b1
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spelling	oai:doaj.org-article:91895aacf521486384904a7e5e5bb7b12021-12-02T17:38:21ZAntibacterial susceptability of E. coli strains isolated from raw milk10.38045/ohrm.2021.3.082887-34582587-3466https://doaj.org/article/91895aacf521486384904a7e5e5bb7b12021-06-01T00:00:00Zhttps://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/139/97https://doaj.org/toc/2887-3458https://doaj.org/toc/2587-3466Introduction. The processing of most raw milk products can lead to contamination with unwanted microflora due to poor sanitation and hygienic conditions. The inadequate antibiotic use over the past decades has led to the emergence and wide spread of bacterial populations, particularly of Escherichia coli, which developed resistance to antibacterial drugs. Material and methods. Raw milk samples were obtained from clinically healthy cows on farms from Kiev and Poltava regions to identify E . coli, Staphylococcus spp., Enterococcus spp. isolates. Antimicrobial susceptibility testing was performed using the EUCAST disk diffusion method and MU on “Determination of microbial susceptibility to antibacterial drugs”. Results. The examined milk samples revealed the presence of E . coli, Staphylococcus spp. and Enterococcus spp. isolates, which proves poor sanitary and hygienic conditions of milk production process. Escherichia coli isolates were found susceptible to Ampicillin/sulbactam, Cefoxitin (100%), Meropenem, Tobramycin (100%), Netilin, Tigecycline, Nitroxoline (100%), Gatifloxacin, and Nitrofurantoin (100%). The studied E. coli isolates were found resistant to Ampicillin (100%), Imipenem, Tetracycline, and Doxycycline (100%). 41.7% of isolates of Staphylococcus epidermidis, Staphylococcus aureus were found resistant to Oxacillin, of which 90% were resistant to Benzylpenicillin and 20% to Rifampicin. Conclusions. The circulation of antibiotic-resistant Enterobacteriaceae strains among farm animals is a major problem requiring a strategy development aimed to prevent the emergence and spread of antibiotic resistance worldwide.Valerii USHKALOVVyacheslav DANCHUKArtem USHKALOV Aidyn SALMANOVYuriy VISHOVANSergiy BOIANOVSKIYSergiy TERESHCHENKOLiliana DAVYDOVSKAAsociatia de Biosiguranta si Biosecuritatearticleescherichia colistrainsmicroorganismsraw milkantibiotic resistancesusceptibilityMedicineRScienceQENFRRORUOne Health & Risk Management, Vol 2, Iss 3, Pp 48-54 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR RO RU
topic	escherichia coli strains microorganisms raw milk antibiotic resistance susceptibility Medicine R Science Q
spellingShingle	escherichia coli strains microorganisms raw milk antibiotic resistance susceptibility Medicine R Science Q Valerii USHKALOV Vyacheslav DANCHUK Artem USHKALOV Aidyn SALMANOV Yuriy VISHOVAN Sergiy BOIANOVSKIY Sergiy TERESHCHENKO Liliana DAVYDOVSKA Antibacterial susceptability of E. coli strains isolated from raw milk
description	Introduction. The processing of most raw milk products can lead to contamination with unwanted microflora due to poor sanitation and hygienic conditions. The inadequate antibiotic use over the past decades has led to the emergence and wide spread of bacterial populations, particularly of Escherichia coli, which developed resistance to antibacterial drugs. Material and methods. Raw milk samples were obtained from clinically healthy cows on farms from Kiev and Poltava regions to identify E . coli, Staphylococcus spp., Enterococcus spp. isolates. Antimicrobial susceptibility testing was performed using the EUCAST disk diffusion method and MU on “Determination of microbial susceptibility to antibacterial drugs”. Results. The examined milk samples revealed the presence of E . coli, Staphylococcus spp. and Enterococcus spp. isolates, which proves poor sanitary and hygienic conditions of milk production process. Escherichia coli isolates were found susceptible to Ampicillin/sulbactam, Cefoxitin (100%), Meropenem, Tobramycin (100%), Netilin, Tigecycline, Nitroxoline (100%), Gatifloxacin, and Nitrofurantoin (100%). The studied E. coli isolates were found resistant to Ampicillin (100%), Imipenem, Tetracycline, and Doxycycline (100%). 41.7% of isolates of Staphylococcus epidermidis, Staphylococcus aureus were found resistant to Oxacillin, of which 90% were resistant to Benzylpenicillin and 20% to Rifampicin. Conclusions. The circulation of antibiotic-resistant Enterobacteriaceae strains among farm animals is a major problem requiring a strategy development aimed to prevent the emergence and spread of antibiotic resistance worldwide.
format	article
author	Valerii USHKALOV Vyacheslav DANCHUK Artem USHKALOV Aidyn SALMANOV Yuriy VISHOVAN Sergiy BOIANOVSKIY Sergiy TERESHCHENKO Liliana DAVYDOVSKA
author_facet	Valerii USHKALOV Vyacheslav DANCHUK Artem USHKALOV Aidyn SALMANOV Yuriy VISHOVAN Sergiy BOIANOVSKIY Sergiy TERESHCHENKO Liliana DAVYDOVSKA
author_sort	Valerii USHKALOV
title	Antibacterial susceptability of E. coli strains isolated from raw milk
title_short	Antibacterial susceptability of E. coli strains isolated from raw milk
title_full	Antibacterial susceptability of E. coli strains isolated from raw milk
title_fullStr	Antibacterial susceptability of E. coli strains isolated from raw milk
title_full_unstemmed	Antibacterial susceptability of E. coli strains isolated from raw milk
title_sort	antibacterial susceptability of e. coli strains isolated from raw milk
publisher	Asociatia de Biosiguranta si Biosecuritate
publishDate	2021
url	https://doaj.org/article/91895aacf521486384904a7e5e5bb7b1
work_keys_str_mv	AT valeriiushkalov antibacterialsusceptabilityofecolistrainsisolatedfromrawmilk AT vyacheslavdanchuk antibacterialsusceptabilityofecolistrainsisolatedfromrawmilk AT artemushkalov antibacterialsusceptabilityofecolistrainsisolatedfromrawmilk AT aidynsalmanov antibacterialsusceptabilityofecolistrainsisolatedfromrawmilk AT yuriyvishovan antibacterialsusceptabilityofecolistrainsisolatedfromrawmilk AT sergiyboianovskiy antibacterialsusceptabilityofecolistrainsisolatedfromrawmilk AT sergiytereshchenko antibacterialsusceptabilityofecolistrainsisolatedfromrawmilk AT lilianadavydovska antibacterialsusceptabilityofecolistrainsisolatedfromrawmilk
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Antibacterial susceptability of E. coli strains isolated from raw milk

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