Black Bean (<i>Phaseolus vulgaris</i> L.) Polyphenolic Extract Exerts Antioxidant and Antiaging Potential

Phenolic compounds present in common beans (<i>Phaseolus vulgaris</i> L.) have been reported to possess antimicrobial, anti-inflammatory and ultraviolet radiation (UVR) protective properties. UVR from sunlight, which consists of UV-B and UV-A radiations, induces reactive oxygen species (...

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Autores principales:	David Fonseca-Hernández, Eugenia Del Carmen Lugo-Cervantes, Antonio Escobedo-Reyes, Luis Mojica
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	phenolic compounds black bean tyrosinase elastase antioxidant supercritical fluids extraction Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/b910a49030f840d19f8cfc6ba8535494
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spelling	oai:doaj.org-article:b910a49030f840d19f8cfc6ba85354942021-11-11T18:39:32ZBlack Bean (<i>Phaseolus vulgaris</i> L.) Polyphenolic Extract Exerts Antioxidant and Antiaging Potential10.3390/molecules262167161420-3049https://doaj.org/article/b910a49030f840d19f8cfc6ba85354942021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6716https://doaj.org/toc/1420-3049Phenolic compounds present in common beans (<i>Phaseolus vulgaris</i> L.) have been reported to possess antimicrobial, anti-inflammatory and ultraviolet radiation (UVR) protective properties. UVR from sunlight, which consists of UV-B and UV-A radiations, induces reactive oxygen species (ROS) and free radical formation, consequently activating proteinases and enzymes such as elastase and tyrosinase, leading to premature skin aging. The objective of this work was to extract, characterize and evaluate the antioxidant and antiaging potential of polyphenols from a black bean endemic variety. The polyphenolic extract was obtained from black beans by supercritical fluid extraction (SFE) using CO<sub>2</sub> with a mixture of water–ethanol as a cosolvent and conventional leaching with a mixture of water–ethanol as solvent. The polyphenolic extracts were purified and characterized, and antioxidant potential, tyrosinase and elastase inhibitory potentials were measured. The extract obtained using the SFE method using CO<sub>2</sub> and H<sub>2</sub>O–Ethanol (50:50 <i>v</i>/<i>v</i>) as a cosolvent showed the highest total phenolic compounds yield, with 66.60 ± 7.41 mg GAE/g coat (<i>p</i> > 0.05) and 7.30 ± 0.64 mg C3GE/g coat (<i>p</i> < 0.05) of anthocyanins compared to conventional leaching. Nineteen tentative phenolic compounds were identified in leaching crude extract using ESI-QTOF. Quercetin-3-D-galactoside was identified in crude and purified extracts. The purified SFC extract showed IC<sub>50</sub> 0.05 ± 0.002 and IC<sub>50</sub> 0.21 ± 0.008 mg/mL for DPPH and ABTS, respectively. The lowest IC<sub>50</sub> value of tyrosinase inhibition was 0.143 ± 0.02 mg/mL and 0.005 ± 0.003 mg/mL of elastase inhibition for leaching purified extract. Phenolic compounds presented theoretical free energy values ranging from −5.3 to −7.8 kcal/mol for tyrosinase and −2.5 to −6.8 kcal/mol for elastase in molecular docking (in silico) studies. The results suggest that the purified extracts obtained by SFE or conventional leaching extraction could act as antioxidant and antiaging ingredients for cosmeceutical applications.David Fonseca-HernándezEugenia Del Carmen Lugo-CervantesAntonio Escobedo-ReyesLuis MojicaMDPI AGarticlephenolic compoundsblack beantyrosinaseelastaseantioxidantsupercritical fluids extractionOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6716, p 6716 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	phenolic compounds black bean tyrosinase elastase antioxidant supercritical fluids extraction Organic chemistry QD241-441
spellingShingle	phenolic compounds black bean tyrosinase elastase antioxidant supercritical fluids extraction Organic chemistry QD241-441 David Fonseca-Hernández Eugenia Del Carmen Lugo-Cervantes Antonio Escobedo-Reyes Luis Mojica Black Bean (<i>Phaseolus vulgaris</i> L.) Polyphenolic Extract Exerts Antioxidant and Antiaging Potential
description	Phenolic compounds present in common beans (<i>Phaseolus vulgaris</i> L.) have been reported to possess antimicrobial, anti-inflammatory and ultraviolet radiation (UVR) protective properties. UVR from sunlight, which consists of UV-B and UV-A radiations, induces reactive oxygen species (ROS) and free radical formation, consequently activating proteinases and enzymes such as elastase and tyrosinase, leading to premature skin aging. The objective of this work was to extract, characterize and evaluate the antioxidant and antiaging potential of polyphenols from a black bean endemic variety. The polyphenolic extract was obtained from black beans by supercritical fluid extraction (SFE) using CO<sub>2</sub> with a mixture of water–ethanol as a cosolvent and conventional leaching with a mixture of water–ethanol as solvent. The polyphenolic extracts were purified and characterized, and antioxidant potential, tyrosinase and elastase inhibitory potentials were measured. The extract obtained using the SFE method using CO<sub>2</sub> and H<sub>2</sub>O–Ethanol (50:50 <i>v</i>/<i>v</i>) as a cosolvent showed the highest total phenolic compounds yield, with 66.60 ± 7.41 mg GAE/g coat (<i>p</i> > 0.05) and 7.30 ± 0.64 mg C3GE/g coat (<i>p</i> < 0.05) of anthocyanins compared to conventional leaching. Nineteen tentative phenolic compounds were identified in leaching crude extract using ESI-QTOF. Quercetin-3-D-galactoside was identified in crude and purified extracts. The purified SFC extract showed IC<sub>50</sub> 0.05 ± 0.002 and IC<sub>50</sub> 0.21 ± 0.008 mg/mL for DPPH and ABTS, respectively. The lowest IC<sub>50</sub> value of tyrosinase inhibition was 0.143 ± 0.02 mg/mL and 0.005 ± 0.003 mg/mL of elastase inhibition for leaching purified extract. Phenolic compounds presented theoretical free energy values ranging from −5.3 to −7.8 kcal/mol for tyrosinase and −2.5 to −6.8 kcal/mol for elastase in molecular docking (in silico) studies. The results suggest that the purified extracts obtained by SFE or conventional leaching extraction could act as antioxidant and antiaging ingredients for cosmeceutical applications.
format	article
author	David Fonseca-Hernández Eugenia Del Carmen Lugo-Cervantes Antonio Escobedo-Reyes Luis Mojica
author_facet	David Fonseca-Hernández Eugenia Del Carmen Lugo-Cervantes Antonio Escobedo-Reyes Luis Mojica
author_sort	David Fonseca-Hernández
title	Black Bean (<i>Phaseolus vulgaris</i> L.) Polyphenolic Extract Exerts Antioxidant and Antiaging Potential
title_short	Black Bean (<i>Phaseolus vulgaris</i> L.) Polyphenolic Extract Exerts Antioxidant and Antiaging Potential
title_full	Black Bean (<i>Phaseolus vulgaris</i> L.) Polyphenolic Extract Exerts Antioxidant and Antiaging Potential
title_fullStr	Black Bean (<i>Phaseolus vulgaris</i> L.) Polyphenolic Extract Exerts Antioxidant and Antiaging Potential
title_full_unstemmed	Black Bean (<i>Phaseolus vulgaris</i> L.) Polyphenolic Extract Exerts Antioxidant and Antiaging Potential
title_sort	black bean (<i>phaseolus vulgaris</i> l.) polyphenolic extract exerts antioxidant and antiaging potential
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b910a49030f840d19f8cfc6ba8535494
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Black Bean (<i>Phaseolus vulgaris</i> L.) Polyphenolic Extract Exerts Antioxidant and Antiaging Potential

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