Role of PPE18 protein in intracellular survival and pathogenicity of Mycobacterium tuberculosis in mice.

<h4>Background</h4>Ever since its discovery the mycobacterial proline-proline-glutamic acid (PPE) family of proteins has generated a huge amount of interest. Understanding the role of these proteins in the pathogenesis of Mycobacterium tuberculosis (Mtb) is important. We have demonstrate...

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Autores principales:	Khalid Hussain Bhat, Asma Ahmed, Santosh Kumar, Pawan Sharma, Sangita Mukhopadhyay
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c0ba75636d054745bb65d86bad8c25ac
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spelling	oai:doaj.org-article:c0ba75636d054745bb65d86bad8c25ac2021-11-18T08:03:09ZRole of PPE18 protein in intracellular survival and pathogenicity of Mycobacterium tuberculosis in mice.1932-620310.1371/journal.pone.0052601https://doaj.org/article/c0ba75636d054745bb65d86bad8c25ac2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23300718/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Ever since its discovery the mycobacterial proline-proline-glutamic acid (PPE) family of proteins has generated a huge amount of interest. Understanding the role of these proteins in the pathogenesis of Mycobacterium tuberculosis (Mtb) is important. We have demonstrated earlier that the PPE18 protein of Mtb induces IL-10 production in macrophages with subsequent downregulation of pro-inflammatory cytokines like IL-12 and TNF-α and favors a T-helper (Th) 2-type of immune response.<h4>Methodology/principal findings</h4>Using a ppe18 genetic knock-out Mtb strain, we have now carried out infection studies in mice to understand the role of PPE18 in Mtb virulence. The studies reveal that lack of PPE18 leads to attenuation of Mtb in vivo. Mice infected with the ppe18 deleted strain have reduced infection burden in lung, liver and spleen and have better survival rates compared to mice infected with the wild-type Mtb strain.<h4>Conclusions/significance</h4>Taken together our data suggest that PPE18 could be a crucial virulence factor for intracellular survival of Mtb.Khalid Hussain BhatAsma AhmedSantosh KumarPawan SharmaSangita MukhopadhyayPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52601 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Khalid Hussain Bhat Asma Ahmed Santosh Kumar Pawan Sharma Sangita Mukhopadhyay Role of PPE18 protein in intracellular survival and pathogenicity of Mycobacterium tuberculosis in mice.
description	<h4>Background</h4>Ever since its discovery the mycobacterial proline-proline-glutamic acid (PPE) family of proteins has generated a huge amount of interest. Understanding the role of these proteins in the pathogenesis of Mycobacterium tuberculosis (Mtb) is important. We have demonstrated earlier that the PPE18 protein of Mtb induces IL-10 production in macrophages with subsequent downregulation of pro-inflammatory cytokines like IL-12 and TNF-α and favors a T-helper (Th) 2-type of immune response.<h4>Methodology/principal findings</h4>Using a ppe18 genetic knock-out Mtb strain, we have now carried out infection studies in mice to understand the role of PPE18 in Mtb virulence. The studies reveal that lack of PPE18 leads to attenuation of Mtb in vivo. Mice infected with the ppe18 deleted strain have reduced infection burden in lung, liver and spleen and have better survival rates compared to mice infected with the wild-type Mtb strain.<h4>Conclusions/significance</h4>Taken together our data suggest that PPE18 could be a crucial virulence factor for intracellular survival of Mtb.
format	article
author	Khalid Hussain Bhat Asma Ahmed Santosh Kumar Pawan Sharma Sangita Mukhopadhyay
author_facet	Khalid Hussain Bhat Asma Ahmed Santosh Kumar Pawan Sharma Sangita Mukhopadhyay
author_sort	Khalid Hussain Bhat
title	Role of PPE18 protein in intracellular survival and pathogenicity of Mycobacterium tuberculosis in mice.
title_short	Role of PPE18 protein in intracellular survival and pathogenicity of Mycobacterium tuberculosis in mice.
title_full	Role of PPE18 protein in intracellular survival and pathogenicity of Mycobacterium tuberculosis in mice.
title_fullStr	Role of PPE18 protein in intracellular survival and pathogenicity of Mycobacterium tuberculosis in mice.
title_full_unstemmed	Role of PPE18 protein in intracellular survival and pathogenicity of Mycobacterium tuberculosis in mice.
title_sort	role of ppe18 protein in intracellular survival and pathogenicity of mycobacterium tuberculosis in mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c0ba75636d054745bb65d86bad8c25ac
work_keys_str_mv	AT khalidhussainbhat roleofppe18proteininintracellularsurvivalandpathogenicityofmycobacteriumtuberculosisinmice AT asmaahmed roleofppe18proteininintracellularsurvivalandpathogenicityofmycobacteriumtuberculosisinmice AT santoshkumar roleofppe18proteininintracellularsurvivalandpathogenicityofmycobacteriumtuberculosisinmice AT pawansharma roleofppe18proteininintracellularsurvivalandpathogenicityofmycobacteriumtuberculosisinmice AT sangitamukhopadhyay roleofppe18proteininintracellularsurvivalandpathogenicityofmycobacteriumtuberculosisinmice
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Role of PPE18 protein in intracellular survival and pathogenicity of Mycobacterium tuberculosis in mice.

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