Specialized Transduction Designed for Precise High-Throughput Unmarked Deletions in <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>

ABSTRACT Specialized transduction has proven to be useful for generating deletion mutants in most mycobacteria, including virulent Mycobacterium tuberculosis. We have improved this system by developing (i) a single-step strategy for the construction of allelic exchange substrates (AES), (ii) a tempe...

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Bibliographic Details
Main Authors: Paras Jain, Tsungda Hsu, Masayoshi Arai, Karolin Biermann, David S. Thaler, Andrew Nguyen, Pablo A. González, Joann M. Tufariello, Jordan Kriakov, Bing Chen, Michelle H. Larsen, William R. Jacobs
Format: article
Language:EN
Published: American Society for Microbiology 2014
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Online Access:https://doaj.org/article/c26cd4668a5142cca824b53be638ee1f
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