Specialized Transduction Designed for Precise High-Throughput Unmarked Deletions in <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
ABSTRACT Specialized transduction has proven to be useful for generating deletion mutants in most mycobacteria, including virulent Mycobacterium tuberculosis. We have improved this system by developing (i) a single-step strategy for the construction of allelic exchange substrates (AES), (ii) a tempe...
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Auteurs principaux: | , , , , , , , , , , , |
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Format: | article |
Langue: | EN |
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American Society for Microbiology
2014
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Accès en ligne: | https://doaj.org/article/c26cd4668a5142cca824b53be638ee1f |
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