What’s in a Name? Species-Wide Whole-Genome Sequencing Resolves Invasive and Noninvasive Lineages of <named-content content-type="genus-species">Salmonella enterica</named-content> Serotype Paratyphi B

ABSTRACT For 100 years, it has been obvious that Salmonella enterica strains sharing the serotype with the formula 1,4,[5],12:b:1,2—now known as Paratyphi B—can cause diseases ranging from serious systemic infections to self-limiting gastroenteritis. Despite considerable predicted diversity between...

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Autores principales:	Thomas R. Connor, Sian V. Owen, Gemma Langridge, Steve Connell, Satheesh Nair, Sandra Reuter, Timothy J. Dallman, Jukka Corander, Kristine C. Tabing, Simon Le Hello, Maria Fookes, Benoît Doublet, Zhemin Zhou, Theresa Feltwell, Matthew J. Ellington, Silvia Herrera, Matthew Gilmour, Axel Cloeckaert, Mark Achtman, Julian Parkhill, John Wain, Elizabeth De Pinna, François-Xavier Weill, Tansy Peters, Nick Thomson
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Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c7ccd8edb6b2430a9cea44bc8c14c6d5
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spelling	oai:doaj.org-article:c7ccd8edb6b2430a9cea44bc8c14c6d52021-11-15T15:50:18ZWhat’s in a Name? Species-Wide Whole-Genome Sequencing Resolves Invasive and Noninvasive Lineages of <named-content content-type="genus-species">Salmonella enterica</named-content> Serotype Paratyphi B10.1128/mBio.00527-162150-7511https://doaj.org/article/c7ccd8edb6b2430a9cea44bc8c14c6d52016-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00527-16https://doaj.org/toc/2150-7511ABSTRACT For 100 years, it has been obvious that Salmonella enterica strains sharing the serotype with the formula 1,4,[5],12:b:1,2—now known as Paratyphi B—can cause diseases ranging from serious systemic infections to self-limiting gastroenteritis. Despite considerable predicted diversity between strains carrying the common Paratyphi B serotype, there remain few methods that subdivide the group into groups that are congruent with their disease phenotypes. Paratyphi B therefore represents one of the canonical examples in Salmonella where serotyping combined with classical microbiological tests fails to provide clinically informative information. Here, we use genomics to provide the first high-resolution view of this serotype, placing it into a wider genomic context of the Salmonella enterica species. These analyses reveal why it has been impossible to subdivide this serotype based upon phenotypic and limited molecular approaches. By examining the genomic data in detail, we are able to identify common features that correlate with strains of clinical importance. The results presented here provide new diagnostic targets, as well as posing important new questions about the basis for the invasive disease phenotype observed in a subset of strains. IMPORTANCE Salmonella enterica strains carrying the serotype Paratyphi B have long been known to possess Jekyll and Hyde characteristics; some cause gastroenteritis, while others cause serious invasive disease. Understanding what makes up the population of strains carrying this serotype, as well as the source of their invasive disease, is a 100-year-old puzzle that we address here using genomics. Our analysis provides the first high-resolution view of this serotype, placing strains carrying serotype Paratyphi B into the wider genomic context of the Salmonella enterica species. This work reveals a history of disease dating back to the middle ages, caused by a group of distinct lineages with various abilities to cause invasive disease. By quantifying the key genomic differences between the invasive and noninvasive populations, we are able to identify key virulence-related targets that can form the basis of simple, rapid, point-of-care tests.Thomas R. ConnorSian V. OwenGemma LangridgeSteve ConnellSatheesh NairSandra ReuterTimothy J. DallmanJukka CoranderKristine C. TabingSimon Le HelloMaria FookesBenoît DoubletZhemin ZhouTheresa FeltwellMatthew J. EllingtonSilvia HerreraMatthew GilmourAxel CloeckaertMark AchtmanJulian ParkhillJohn WainElizabeth De PinnaFrançois-Xavier WeillTansy PetersNick ThomsonAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 4 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Thomas R. Connor Sian V. Owen Gemma Langridge Steve Connell Satheesh Nair Sandra Reuter Timothy J. Dallman Jukka Corander Kristine C. Tabing Simon Le Hello Maria Fookes Benoît Doublet Zhemin Zhou Theresa Feltwell Matthew J. Ellington Silvia Herrera Matthew Gilmour Axel Cloeckaert Mark Achtman Julian Parkhill John Wain Elizabeth De Pinna François-Xavier Weill Tansy Peters Nick Thomson What’s in a Name? Species-Wide Whole-Genome Sequencing Resolves Invasive and Noninvasive Lineages of <named-content content-type="genus-species">Salmonella enterica</named-content> Serotype Paratyphi B
description	ABSTRACT For 100 years, it has been obvious that Salmonella enterica strains sharing the serotype with the formula 1,4,[5],12:b:1,2—now known as Paratyphi B—can cause diseases ranging from serious systemic infections to self-limiting gastroenteritis. Despite considerable predicted diversity between strains carrying the common Paratyphi B serotype, there remain few methods that subdivide the group into groups that are congruent with their disease phenotypes. Paratyphi B therefore represents one of the canonical examples in Salmonella where serotyping combined with classical microbiological tests fails to provide clinically informative information. Here, we use genomics to provide the first high-resolution view of this serotype, placing it into a wider genomic context of the Salmonella enterica species. These analyses reveal why it has been impossible to subdivide this serotype based upon phenotypic and limited molecular approaches. By examining the genomic data in detail, we are able to identify common features that correlate with strains of clinical importance. The results presented here provide new diagnostic targets, as well as posing important new questions about the basis for the invasive disease phenotype observed in a subset of strains. IMPORTANCE Salmonella enterica strains carrying the serotype Paratyphi B have long been known to possess Jekyll and Hyde characteristics; some cause gastroenteritis, while others cause serious invasive disease. Understanding what makes up the population of strains carrying this serotype, as well as the source of their invasive disease, is a 100-year-old puzzle that we address here using genomics. Our analysis provides the first high-resolution view of this serotype, placing strains carrying serotype Paratyphi B into the wider genomic context of the Salmonella enterica species. This work reveals a history of disease dating back to the middle ages, caused by a group of distinct lineages with various abilities to cause invasive disease. By quantifying the key genomic differences between the invasive and noninvasive populations, we are able to identify key virulence-related targets that can form the basis of simple, rapid, point-of-care tests.
format	article
author	Thomas R. Connor Sian V. Owen Gemma Langridge Steve Connell Satheesh Nair Sandra Reuter Timothy J. Dallman Jukka Corander Kristine C. Tabing Simon Le Hello Maria Fookes Benoît Doublet Zhemin Zhou Theresa Feltwell Matthew J. Ellington Silvia Herrera Matthew Gilmour Axel Cloeckaert Mark Achtman Julian Parkhill John Wain Elizabeth De Pinna François-Xavier Weill Tansy Peters Nick Thomson
author_facet	Thomas R. Connor Sian V. Owen Gemma Langridge Steve Connell Satheesh Nair Sandra Reuter Timothy J. Dallman Jukka Corander Kristine C. Tabing Simon Le Hello Maria Fookes Benoît Doublet Zhemin Zhou Theresa Feltwell Matthew J. Ellington Silvia Herrera Matthew Gilmour Axel Cloeckaert Mark Achtman Julian Parkhill John Wain Elizabeth De Pinna François-Xavier Weill Tansy Peters Nick Thomson
author_sort	Thomas R. Connor
title	What’s in a Name? Species-Wide Whole-Genome Sequencing Resolves Invasive and Noninvasive Lineages of <named-content content-type="genus-species">Salmonella enterica</named-content> Serotype Paratyphi B
title_short	What’s in a Name? Species-Wide Whole-Genome Sequencing Resolves Invasive and Noninvasive Lineages of <named-content content-type="genus-species">Salmonella enterica</named-content> Serotype Paratyphi B
title_full	What’s in a Name? Species-Wide Whole-Genome Sequencing Resolves Invasive and Noninvasive Lineages of <named-content content-type="genus-species">Salmonella enterica</named-content> Serotype Paratyphi B
title_fullStr	What’s in a Name? Species-Wide Whole-Genome Sequencing Resolves Invasive and Noninvasive Lineages of <named-content content-type="genus-species">Salmonella enterica</named-content> Serotype Paratyphi B
title_full_unstemmed	What’s in a Name? Species-Wide Whole-Genome Sequencing Resolves Invasive and Noninvasive Lineages of <named-content content-type="genus-species">Salmonella enterica</named-content> Serotype Paratyphi B
title_sort	what’s in a name? species-wide whole-genome sequencing resolves invasive and noninvasive lineages of <named-content content-type="genus-species">salmonella enterica</named-content> serotype paratyphi b
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/c7ccd8edb6b2430a9cea44bc8c14c6d5
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What’s in a Name? Species-Wide Whole-Genome Sequencing Resolves Invasive and Noninvasive Lineages of <named-content content-type="genus-species">Salmonella enterica</named-content> Serotype Paratyphi B

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