Molecular Detection and Characterization of Intestinal and Blood Parasites in Wild Chimpanzees (<i>Pan troglodytes verus</i>) in Senegal

Wild chimpanzee populations in West Africa (<i>Pan troglodytes verus</i>) have dramatically decreased as a direct consequence of anthropogenic activities and infectious diseases. Little information is currently available on the epidemiology, pathogenic significance, and zoonotic potentia...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Pamela C. Köster, Justinn Renelies-Hamilton, Laia Dotras, Manuel Llana, Celia Vinagre-Izquierdo, Petras Prakas, Donatas Sneideris, Alejandro Dashti, Begoña Bailo, Marta Lanza, Alejandra Jiménez-Mejías, Carlota Muñoz-García, Aly S. Muadica, David González-Barrio, José M. Rubio, Isabel Fuentes, Francisco Ponce-Gordo, Rafael Calero-Bernal, David Carmena
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
PCR
Acceso en línea:https://doaj.org/article/cf6340cd124040f18467cbeeb0428e2c
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:cf6340cd124040f18467cbeeb0428e2c
record_format dspace
spelling oai:doaj.org-article:cf6340cd124040f18467cbeeb0428e2c2021-11-25T16:20:39ZMolecular Detection and Characterization of Intestinal and Blood Parasites in Wild Chimpanzees (<i>Pan troglodytes verus</i>) in Senegal10.3390/ani111132912076-2615https://doaj.org/article/cf6340cd124040f18467cbeeb0428e2c2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3291https://doaj.org/toc/2076-2615Wild chimpanzee populations in West Africa (<i>Pan troglodytes verus</i>) have dramatically decreased as a direct consequence of anthropogenic activities and infectious diseases. Little information is currently available on the epidemiology, pathogenic significance, and zoonotic potential of protist species in wild chimpanzees. This study investigates the occurrence and genetic diversity of intestinal and blood protists as well as filariae in faecal samples (<i>n</i> = 234) from wild chimpanzees in the Dindefelo Community Nature Reserve, Senegal. PCR-based results revealed the presence of intestinal potential pathogens (<i>Sarcocystis</i> spp.: 11.5%; <i>Giardia duodenalis</i>: 2.1%; <i>Cryptosporidium hominis</i>: 0.9%), protist of uncertain pathogenicity (<i>Blastocystis</i> sp.: 5.6%), and commensal species (<i>Entamoeba dispar</i>: 18.4%; <i>Troglodytella abrassarti</i>: 5.6%). <i>Entamoeba histolytica</i>, <i>Enterocytozoon bieneusi</i>, and <i>Balantioides coli</i> were undetected. Blood protists including <i>Plasmodium malariae</i> (0.4%), <i>Trypanosoma brucei</i> (1.3%), and <i>Mansonella perstans</i> (9.8%) were also identified. Sanger sequencing analyses revealed host-adapted genetic variants within <i>Blastocystis</i>, but other parasitic pathogens (<i>C. hominis</i>, <i>P. malariae</i>, <i>T. brucei</i>, <i>M. perstans</i>) have zoonotic potential, suggesting that cross-species transmission between wild chimpanzees and humans is possible in areas where both species overlap. Additionally, we explored potential interactions between intestinal/blood protist species and seasonality and climate variables. Chimpanzees seem to play a more complex role on the epidemiology of pathogenic and commensal protist and nematode species than initially anticipated.Pamela C. KösterJustinn Renelies-HamiltonLaia DotrasManuel LlanaCelia Vinagre-IzquierdoPetras PrakasDonatas SneiderisAlejandro DashtiBegoña BailoMarta LanzaAlejandra Jiménez-MejíasCarlota Muñoz-GarcíaAly S. MuadicaDavid González-BarrioJosé M. RubioIsabel FuentesFrancisco Ponce-GordoRafael Calero-BernalDavid CarmenaMDPI AGarticleprotistsnon-human primatesendangeredconservationPCRgenotypingVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3291, p 3291 (2021)
institution DOAJ
collection DOAJ
language EN
topic protists
non-human primates
endangered
conservation
PCR
genotyping
Veterinary medicine
SF600-1100
Zoology
QL1-991
spellingShingle protists
non-human primates
endangered
conservation
PCR
genotyping
Veterinary medicine
SF600-1100
Zoology
QL1-991
Pamela C. Köster
Justinn Renelies-Hamilton
Laia Dotras
Manuel Llana
Celia Vinagre-Izquierdo
Petras Prakas
Donatas Sneideris
Alejandro Dashti
Begoña Bailo
Marta Lanza
Alejandra Jiménez-Mejías
Carlota Muñoz-García
Aly S. Muadica
David González-Barrio
José M. Rubio
Isabel Fuentes
Francisco Ponce-Gordo
Rafael Calero-Bernal
David Carmena
Molecular Detection and Characterization of Intestinal and Blood Parasites in Wild Chimpanzees (<i>Pan troglodytes verus</i>) in Senegal
description Wild chimpanzee populations in West Africa (<i>Pan troglodytes verus</i>) have dramatically decreased as a direct consequence of anthropogenic activities and infectious diseases. Little information is currently available on the epidemiology, pathogenic significance, and zoonotic potential of protist species in wild chimpanzees. This study investigates the occurrence and genetic diversity of intestinal and blood protists as well as filariae in faecal samples (<i>n</i> = 234) from wild chimpanzees in the Dindefelo Community Nature Reserve, Senegal. PCR-based results revealed the presence of intestinal potential pathogens (<i>Sarcocystis</i> spp.: 11.5%; <i>Giardia duodenalis</i>: 2.1%; <i>Cryptosporidium hominis</i>: 0.9%), protist of uncertain pathogenicity (<i>Blastocystis</i> sp.: 5.6%), and commensal species (<i>Entamoeba dispar</i>: 18.4%; <i>Troglodytella abrassarti</i>: 5.6%). <i>Entamoeba histolytica</i>, <i>Enterocytozoon bieneusi</i>, and <i>Balantioides coli</i> were undetected. Blood protists including <i>Plasmodium malariae</i> (0.4%), <i>Trypanosoma brucei</i> (1.3%), and <i>Mansonella perstans</i> (9.8%) were also identified. Sanger sequencing analyses revealed host-adapted genetic variants within <i>Blastocystis</i>, but other parasitic pathogens (<i>C. hominis</i>, <i>P. malariae</i>, <i>T. brucei</i>, <i>M. perstans</i>) have zoonotic potential, suggesting that cross-species transmission between wild chimpanzees and humans is possible in areas where both species overlap. Additionally, we explored potential interactions between intestinal/blood protist species and seasonality and climate variables. Chimpanzees seem to play a more complex role on the epidemiology of pathogenic and commensal protist and nematode species than initially anticipated.
format article
author Pamela C. Köster
Justinn Renelies-Hamilton
Laia Dotras
Manuel Llana
Celia Vinagre-Izquierdo
Petras Prakas
Donatas Sneideris
Alejandro Dashti
Begoña Bailo
Marta Lanza
Alejandra Jiménez-Mejías
Carlota Muñoz-García
Aly S. Muadica
David González-Barrio
José M. Rubio
Isabel Fuentes
Francisco Ponce-Gordo
Rafael Calero-Bernal
David Carmena
author_facet Pamela C. Köster
Justinn Renelies-Hamilton
Laia Dotras
Manuel Llana
Celia Vinagre-Izquierdo
Petras Prakas
Donatas Sneideris
Alejandro Dashti
Begoña Bailo
Marta Lanza
Alejandra Jiménez-Mejías
Carlota Muñoz-García
Aly S. Muadica
David González-Barrio
José M. Rubio
Isabel Fuentes
Francisco Ponce-Gordo
Rafael Calero-Bernal
David Carmena
author_sort Pamela C. Köster
title Molecular Detection and Characterization of Intestinal and Blood Parasites in Wild Chimpanzees (<i>Pan troglodytes verus</i>) in Senegal
title_short Molecular Detection and Characterization of Intestinal and Blood Parasites in Wild Chimpanzees (<i>Pan troglodytes verus</i>) in Senegal
title_full Molecular Detection and Characterization of Intestinal and Blood Parasites in Wild Chimpanzees (<i>Pan troglodytes verus</i>) in Senegal
title_fullStr Molecular Detection and Characterization of Intestinal and Blood Parasites in Wild Chimpanzees (<i>Pan troglodytes verus</i>) in Senegal
title_full_unstemmed Molecular Detection and Characterization of Intestinal and Blood Parasites in Wild Chimpanzees (<i>Pan troglodytes verus</i>) in Senegal
title_sort molecular detection and characterization of intestinal and blood parasites in wild chimpanzees (<i>pan troglodytes verus</i>) in senegal
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/cf6340cd124040f18467cbeeb0428e2c
work_keys_str_mv AT pamelackoster moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT justinnrenelieshamilton moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT laiadotras moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT manuelllana moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT celiavinagreizquierdo moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT petrasprakas moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT donatassneideris moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT alejandrodashti moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT begonabailo moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT martalanza moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT alejandrajimenezmejias moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT carlotamunozgarcia moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT alysmuadica moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT davidgonzalezbarrio moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT josemrubio moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT isabelfuentes moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT franciscoponcegordo moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT rafaelcalerobernal moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
AT davidcarmena moleculardetectionandcharacterizationofintestinalandbloodparasitesinwildchimpanzeesipantroglodytesverusiinsenegal
_version_ 1718413227608506368