Effect of High Hydrostatic Pressure on Stress-Related <i>dnaK</i>, <i>hrcA</i>, and <i>ctsR</i> Expression Patterns in Selected <i>Lactobacilli</i> Strains

Effect of High Hydrostatic Pressure on Stress-Related <i>dnaK</i>, <i>hrcA</i>, and <i>ctsR</i> Expression Patterns in Selected <i>Lactobacilli</i> Strains

Lactic acid bacteria (LAB) in the natural environment meet multiple stressors such as pH and temperature variations, increased nutrition and metabolite concentrations, harmful chemicals, acidic/oxidative conditions, osmotic pressure, and starvation. However, LAB strains are not subjected to high hyd...

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Autores principales: Joanna Bucka-Kolendo, Edyta Juszczuk-Kubiak, Barbara Sokołowska
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
lactic acid bacteria (LAB)
high hydrostatic pressure (HHP)
stress-related genes
gene expression
Genetics
QH426-470
Acceso en línea:https://doaj.org/article/d44b0b050b7f44b899804391fb52a464
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spelling oai:doaj.org-article:d44b0b050b7f44b899804391fb52a4642021-11-25T17:41:14ZEffect of High Hydrostatic Pressure on Stress-Related <i>dnaK</i>, <i>hrcA</i>, and <i>ctsR</i> Expression Patterns in Selected <i>Lactobacilli</i> Strains10.3390/genes121117202073-4425https://doaj.org/article/d44b0b050b7f44b899804391fb52a4642021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1720https://doaj.org/toc/2073-4425Lactic acid bacteria (LAB) in the natural environment meet multiple stressors such as pH and temperature variations, increased nutrition and metabolite concentrations, harmful chemicals, acidic/oxidative conditions, osmotic pressure, and starvation. However, LAB strains are not subjected to high hydrostatic pressure (HHP) which currently is the most common non-thermal decontamination technology in the food industry. In this context, the LAB response to HHP is more difficult to identify compared to other stress-induced responses, and <i>dnaK</i>, <i>ctsR</i>, and <i>hrcA</i> can serve as essential regulators in this reaction. In the present study, the expression level of <i>dnaK</i>, <i>ctsR</i>, and <i>hrcA</i> mRNAs in 15 LAB strains after the HHP (300 MPa/5′) exposure was evaluated. As a result, the HHP-treatment affected the up-regulation of <i>dnaK</i>, <i>ctsR</i>, and <i>hrcA</i> in <i>L. backii</i> KKP 3565, <i>L. backii</i> KKP 3566, <i>L. rhamnosus</i> KKP 3570, <i>L. brevis</i> KKP 3575 strains, whereas, in <i>L. plantarum</i> KKP 3569, <i>L. rhamnosus</i> KKP 3571, <i>L. brevis</i> KKP 3573 all genes were lower expressed. The relative expression level of the <i>dnaK</i>, <i>ctsR</i>, and <i>hrcA</i> either before or after the pressure treatment for <i>L. brevis</i> DSM 6235, <i>L. rhamnosus</i> KKP 3572, <i>L. brevis</i> KKP 3574, <i>L. brevis</i> KKP 3576, <i>L. rossiae</i> KKP 3577, <i>L. curvatus</i> KKP 3578 strains were undetectable. Significant differences in the expression levels were observed, between the control and the HHP treatment strains for <i>dnaK</i> in <i>L. backii</i> KKP 3565, <i>L. backii</i> KKP 3566, <i>L. plantarum</i> KKP 3569, <i>L. rhamnosus</i> KKP 3570, <i>L. rhamnosus</i> KKP 3571, <i>ctsR</i> in, <i>L. backii</i> KKP 3565, <i>L. rhamnosus</i> KKP 3570, <i>L. rhamnosus</i> KKP 3571, and <i>hrcA</i> in <i>L. plantarum</i> KKP 3569, <i>L. rhamnosus</i> KKP 3571. Overall, the studied genes, <i>dnaK</i>, <i>ctsR</i>, and <i>hrcA</i> can be useful markers to indicate the LAB cellular response to HHP. These molecular parameters can help to optimize the desirable LAB growing conditions in industrial processes and to understand the complexity of the stress-related mechanism.Joanna Bucka-KolendoEdyta Juszczuk-KubiakBarbara SokołowskaMDPI AGarticlelactic acid bacteria (LAB)high hydrostatic pressure (HHP)stress-related genesgene expressionGeneticsQH426-470ENGenes, Vol 12, Iss 1720, p 1720 (2021)
institution DOAJ
collection DOAJ
language EN
topic lactic acid bacteria (LAB)
high hydrostatic pressure (HHP)
stress-related genes
gene expression
Genetics
QH426-470
spellingShingle lactic acid bacteria (LAB)
high hydrostatic pressure (HHP)
stress-related genes
gene expression
Genetics
QH426-470
Joanna Bucka-Kolendo
Edyta Juszczuk-Kubiak
Barbara Sokołowska
Effect of High Hydrostatic Pressure on Stress-Related <i>dnaK</i>, <i>hrcA</i>, and <i>ctsR</i> Expression Patterns in Selected <i>Lactobacilli</i> Strains
description Lactic acid bacteria (LAB) in the natural environment meet multiple stressors such as pH and temperature variations, increased nutrition and metabolite concentrations, harmful chemicals, acidic/oxidative conditions, osmotic pressure, and starvation. However, LAB strains are not subjected to high hydrostatic pressure (HHP) which currently is the most common non-thermal decontamination technology in the food industry. In this context, the LAB response to HHP is more difficult to identify compared to other stress-induced responses, and <i>dnaK</i>, <i>ctsR</i>, and <i>hrcA</i> can serve as essential regulators in this reaction. In the present study, the expression level of <i>dnaK</i>, <i>ctsR</i>, and <i>hrcA</i> mRNAs in 15 LAB strains after the HHP (300 MPa/5′) exposure was evaluated. As a result, the HHP-treatment affected the up-regulation of <i>dnaK</i>, <i>ctsR</i>, and <i>hrcA</i> in <i>L. backii</i> KKP 3565, <i>L. backii</i> KKP 3566, <i>L. rhamnosus</i> KKP 3570, <i>L. brevis</i> KKP 3575 strains, whereas, in <i>L. plantarum</i> KKP 3569, <i>L. rhamnosus</i> KKP 3571, <i>L. brevis</i> KKP 3573 all genes were lower expressed. The relative expression level of the <i>dnaK</i>, <i>ctsR</i>, and <i>hrcA</i> either before or after the pressure treatment for <i>L. brevis</i> DSM 6235, <i>L. rhamnosus</i> KKP 3572, <i>L. brevis</i> KKP 3574, <i>L. brevis</i> KKP 3576, <i>L. rossiae</i> KKP 3577, <i>L. curvatus</i> KKP 3578 strains were undetectable. Significant differences in the expression levels were observed, between the control and the HHP treatment strains for <i>dnaK</i> in <i>L. backii</i> KKP 3565, <i>L. backii</i> KKP 3566, <i>L. plantarum</i> KKP 3569, <i>L. rhamnosus</i> KKP 3570, <i>L. rhamnosus</i> KKP 3571, <i>ctsR</i> in, <i>L. backii</i> KKP 3565, <i>L. rhamnosus</i> KKP 3570, <i>L. rhamnosus</i> KKP 3571, and <i>hrcA</i> in <i>L. plantarum</i> KKP 3569, <i>L. rhamnosus</i> KKP 3571. Overall, the studied genes, <i>dnaK</i>, <i>ctsR</i>, and <i>hrcA</i> can be useful markers to indicate the LAB cellular response to HHP. These molecular parameters can help to optimize the desirable LAB growing conditions in industrial processes and to understand the complexity of the stress-related mechanism.
format article
author Joanna Bucka-Kolendo
Edyta Juszczuk-Kubiak
Barbara Sokołowska
author_facet Joanna Bucka-Kolendo
Edyta Juszczuk-Kubiak
Barbara Sokołowska
author_sort Joanna Bucka-Kolendo
title Effect of High Hydrostatic Pressure on Stress-Related <i>dnaK</i>, <i>hrcA</i>, and <i>ctsR</i> Expression Patterns in Selected <i>Lactobacilli</i> Strains
title_short Effect of High Hydrostatic Pressure on Stress-Related <i>dnaK</i>, <i>hrcA</i>, and <i>ctsR</i> Expression Patterns in Selected <i>Lactobacilli</i> Strains
title_full Effect of High Hydrostatic Pressure on Stress-Related <i>dnaK</i>, <i>hrcA</i>, and <i>ctsR</i> Expression Patterns in Selected <i>Lactobacilli</i> Strains
title_fullStr Effect of High Hydrostatic Pressure on Stress-Related <i>dnaK</i>, <i>hrcA</i>, and <i>ctsR</i> Expression Patterns in Selected <i>Lactobacilli</i> Strains
title_full_unstemmed Effect of High Hydrostatic Pressure on Stress-Related <i>dnaK</i>, <i>hrcA</i>, and <i>ctsR</i> Expression Patterns in Selected <i>Lactobacilli</i> Strains
title_sort effect of high hydrostatic pressure on stress-related <i>dnak</i>, <i>hrca</i>, and <i>ctsr</i> expression patterns in selected <i>lactobacilli</i> strains
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/d44b0b050b7f44b899804391fb52a464
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AT barbarasokołowska effectofhighhydrostaticpressureonstressrelatedidnakiihrcaiandictsriexpressionpatternsinselectedilactobacilliistrains
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