In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids

Background: Dietary linoleic (LA) and alpha-linolenic (LN) acids are extensively isomerized and hydrogenated by rumen microbes, and this activity can further contribute to the fatty acid profile of ruminantderived food products. Objective: To evaluate the effects of LA:LN ratio in lipid supplements...

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Autores principales: Julián Andrés Castillo Vargas, Martha Olivera-Ángel, Claúdio Vaz Di Mambro Ribeiro, Edgar Eduardo Daza Caicedo
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Publicado: Universidad de Antioquia 2018
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spelling oai:doaj.org-article:d74989fb82604f1189447cc2c9c1dfe82021-12-01T19:25:55ZIn vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids2256-295810.17533/udea.rccp.v31n3a06https://doaj.org/article/d74989fb82604f1189447cc2c9c1dfe82018-08-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/330535https://doaj.org/toc/2256-2958Background: Dietary linoleic (LA) and alpha-linolenic (LN) acids are extensively isomerized and hydrogenated by rumen microbes, and this activity can further contribute to the fatty acid profile of ruminantderived food products. Objective: To evaluate the effects of LA:LN ratio in lipid supplements on the rumen biohydrogenation kinetics of LA and LN, as well as on the trans-vaccenic acid (VA) production, using an in vitro system. Methods: Rumen fluid was collected from a fistulated steer, diluted with incubation buffer, and then incubated with 500 mg of kikuyu grass (Cenchrus clandestinus) supplemented with 16.3 mg of different LA:LN mixtures (100:0, 75:25, 50:50, 25:75 or 0:100). Incubations were performed in triplicate for a period of 0, 2, 4, 6, 8 or 16 hours. Differences between treatments were evaluated in a completely randomized design. Alternatively, computational chemistry was used to determine the changes in the Gibbs free energy (ΔGrxn) at 39 °C for the principal steps of LA and LN ruminal biohydrogenation. Results: Partial replacement of LA by LN decreased the VA concentration and its accumulation rate; it also increased the stearic acid concentration and the rates of transfer from LA to conjugated linoleic acid (CLA), and from CLA to VA. The conversión from CLA to VA (ΔGrxn = -2.65 kJ/mol) was more spontaneous than that from trans-11, cis-15 octadecadienoic acid (TA) to VA (ΔGrxn = -0.29 kJ/mol). Conclusion: The LA:LN ratio in lipids can modulate LA and LN biohydrogenation (BH) kinetics, as well as the VA production in the rumen.Julián Andrés Castillo VargasMartha Olivera-ÁngelClaúdio Vaz Di Mambro RibeiroEdgar Eduardo Daza CaicedoUniversidad de Antioquiaarticlefatty acidskinetics of biohydrogenationlipid supplementmulti-compartmental modelthermodynamics of biohydrogenationAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 31, Iss 3, Pp 213-222 (2018)
institution DOAJ
collection DOAJ
language EN
topic fatty acids
kinetics of biohydrogenation
lipid supplement
multi-compartmental model
thermodynamics of biohydrogenation
Animal culture
SF1-1100
spellingShingle fatty acids
kinetics of biohydrogenation
lipid supplement
multi-compartmental model
thermodynamics of biohydrogenation
Animal culture
SF1-1100
Julián Andrés Castillo Vargas
Martha Olivera-Ángel
Claúdio Vaz Di Mambro Ribeiro
Edgar Eduardo Daza Caicedo
In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids
description Background: Dietary linoleic (LA) and alpha-linolenic (LN) acids are extensively isomerized and hydrogenated by rumen microbes, and this activity can further contribute to the fatty acid profile of ruminantderived food products. Objective: To evaluate the effects of LA:LN ratio in lipid supplements on the rumen biohydrogenation kinetics of LA and LN, as well as on the trans-vaccenic acid (VA) production, using an in vitro system. Methods: Rumen fluid was collected from a fistulated steer, diluted with incubation buffer, and then incubated with 500 mg of kikuyu grass (Cenchrus clandestinus) supplemented with 16.3 mg of different LA:LN mixtures (100:0, 75:25, 50:50, 25:75 or 0:100). Incubations were performed in triplicate for a period of 0, 2, 4, 6, 8 or 16 hours. Differences between treatments were evaluated in a completely randomized design. Alternatively, computational chemistry was used to determine the changes in the Gibbs free energy (ΔGrxn) at 39 °C for the principal steps of LA and LN ruminal biohydrogenation. Results: Partial replacement of LA by LN decreased the VA concentration and its accumulation rate; it also increased the stearic acid concentration and the rates of transfer from LA to conjugated linoleic acid (CLA), and from CLA to VA. The conversión from CLA to VA (ΔGrxn = -2.65 kJ/mol) was more spontaneous than that from trans-11, cis-15 octadecadienoic acid (TA) to VA (ΔGrxn = -0.29 kJ/mol). Conclusion: The LA:LN ratio in lipids can modulate LA and LN biohydrogenation (BH) kinetics, as well as the VA production in the rumen.
format article
author Julián Andrés Castillo Vargas
Martha Olivera-Ángel
Claúdio Vaz Di Mambro Ribeiro
Edgar Eduardo Daza Caicedo
author_facet Julián Andrés Castillo Vargas
Martha Olivera-Ángel
Claúdio Vaz Di Mambro Ribeiro
Edgar Eduardo Daza Caicedo
author_sort Julián Andrés Castillo Vargas
title In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids
title_short In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids
title_full In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids
title_fullStr In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids
title_full_unstemmed In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids
title_sort in vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids
publisher Universidad de Antioquia
publishDate 2018
url https://doaj.org/article/d74989fb82604f1189447cc2c9c1dfe8
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AT claudiovazdimambroribeiro invitrorumenbiohydrogenationkineticsofmixedlinoleicandalfalinolenicacids
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