Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila

A stoichiometric model for Saccharomyces cerevisiae is reconstructed to analyze the continuous fermentation process of agave juice in Tequila production. The metabolic model contains 94 metabolites and 117 biochemical reactions. From the above set of reactions, 93 of them are linked to internal bioc...

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Autores principales:	José Daniel Padilla-de la-Rosa, Mario Alberto García-Ramírez, Anne Christine Gschaedler-Mathis, Abril Ivette Gómez-Guzmán, Josué R. Solís-Pacheco, Orfil González-Reynoso
Formato:	article
Lenguaje:	EN
Publicado:	AIMS Press 2021
Materias:	flux balance analysis saccharomyces cerevisiae tequila fermentation higher alcohols Biotechnology TP248.13-248.65 Mathematics QA1-939
Acceso en línea:	https://doaj.org/article/dee537d1529a4ac3aa0b93d6760c95ca
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spelling	oai:doaj.org-article:dee537d1529a4ac3aa0b93d6760c95ca2021-11-08T05:44:31ZEstimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila10.3934/mbe.20212591551-0018https://doaj.org/article/dee537d1529a4ac3aa0b93d6760c95ca2021-07-01T00:00:00Zhttps://www.aimspress.com/article/doi/10.3934/mbe.2021259?viewType=HTMLhttps://doaj.org/toc/1551-0018A stoichiometric model for Saccharomyces cerevisiae is reconstructed to analyze the continuous fermentation process of agave juice in Tequila production. The metabolic model contains 94 metabolites and 117 biochemical reactions. From the above set of reactions, 93 of them are linked to internal biochemical reactions and 24 are related to transport fluxes between the medium and the cell. The central metabolism of S. cerevisiae includes the synthesis for 20 amino-acids, carbohydrates, lipids, DNA and RNA. Using flux balance analysis (FBA), different physiological states of S. cerevisiae are shown during the fermentative process; these states are compared with experimental data under different dilution rates (0.04-0.12 h$ ^{-1} $). Moreover, the model performs anabolic and catabolic biochemical reactions for the production of higher alcohols. The importance of the Saccharomyces cerevisiae genomic model in the area of alcoholic beverage fermentation is due to the fact that it allows to estimate the metabolic fluxes during the beverage fermentation process and a physiology state of the microorganism.José Daniel Padilla-de la-RosaMario Alberto García-RamírezAnne Christine Gschaedler-MathisAbril Ivette Gómez-Guzmán Josué R. Solís-PachecoOrfil González-ReynosoAIMS Pressarticleflux balance analysissaccharomyces cerevisiaetequilafermentationhigher alcoholsBiotechnologyTP248.13-248.65MathematicsQA1-939ENMathematical Biosciences and Engineering, Vol 18, Iss 5, Pp 5094-5113 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	flux balance analysis saccharomyces cerevisiae tequila fermentation higher alcohols Biotechnology TP248.13-248.65 Mathematics QA1-939
spellingShingle	flux balance analysis saccharomyces cerevisiae tequila fermentation higher alcohols Biotechnology TP248.13-248.65 Mathematics QA1-939 José Daniel Padilla-de la-Rosa Mario Alberto García-Ramírez Anne Christine Gschaedler-Mathis Abril Ivette Gómez-Guzmán Josué R. Solís-Pacheco Orfil González-Reynoso Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila
description	A stoichiometric model for Saccharomyces cerevisiae is reconstructed to analyze the continuous fermentation process of agave juice in Tequila production. The metabolic model contains 94 metabolites and 117 biochemical reactions. From the above set of reactions, 93 of them are linked to internal biochemical reactions and 24 are related to transport fluxes between the medium and the cell. The central metabolism of S. cerevisiae includes the synthesis for 20 amino-acids, carbohydrates, lipids, DNA and RNA. Using flux balance analysis (FBA), different physiological states of S. cerevisiae are shown during the fermentative process; these states are compared with experimental data under different dilution rates (0.04-0.12 h$ ^{-1} $). Moreover, the model performs anabolic and catabolic biochemical reactions for the production of higher alcohols. The importance of the Saccharomyces cerevisiae genomic model in the area of alcoholic beverage fermentation is due to the fact that it allows to estimate the metabolic fluxes during the beverage fermentation process and a physiology state of the microorganism.
format	article
author	José Daniel Padilla-de la-Rosa Mario Alberto García-Ramírez Anne Christine Gschaedler-Mathis Abril Ivette Gómez-Guzmán Josué R. Solís-Pacheco Orfil González-Reynoso
author_facet	José Daniel Padilla-de la-Rosa Mario Alberto García-Ramírez Anne Christine Gschaedler-Mathis Abril Ivette Gómez-Guzmán Josué R. Solís-Pacheco Orfil González-Reynoso
author_sort	José Daniel Padilla-de la-Rosa
title	Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila
title_short	Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila
title_full	Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila
title_fullStr	Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila
title_full_unstemmed	Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila
title_sort	estimation of metabolic fluxes distribution in saccharomyces cerevisiae during the production of volatile compounds of tequila
publisher	AIMS Press
publishDate	2021
url	https://doaj.org/article/dee537d1529a4ac3aa0b93d6760c95ca
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Estimation of metabolic fluxes distribution in Saccharomyces cerevisiae during the production of volatile compounds of Tequila

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