Prevalence, Molecular Characterization and Antimicrobial Susceptibility of Clostridioides difficile Isolated from Pig Carcasses and Pork Products in Central Italy

In the last decade, the incidence and severity of Clostridioides difficile infections (CDIs) in humans have been increasing and community-associated infections have been described. For these reasons, the interest in C. difficile in food and in food animals has i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Caterina Licciardi, Sara Primavilla, Rossana Roila, Alessia Lupattelli, Silvana Farneti, Giuliana Blasi, Annalisa Petruzzelli, Ilenia Drigo, Enrico Di Raimo Marrocchi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Clostridioides difficile</i> pig carcasses pork products food safety real-time PCR ribotype Medicine R
Acceso en línea:	https://doaj.org/article/f8d28f1f3cb8478d9a65f1c37f93f492
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f8d28f1f3cb8478d9a65f1c37f93f492
record_format	dspace
spelling	oai:doaj.org-article:f8d28f1f3cb8478d9a65f1c37f93f4922021-11-11T16:29:51ZPrevalence, Molecular Characterization and Antimicrobial Susceptibility of <i>Clostridioides difficile</i> Isolated from Pig Carcasses and Pork Products in Central Italy10.3390/ijerph1821113681660-46011661-7827https://doaj.org/article/f8d28f1f3cb8478d9a65f1c37f93f4922021-10-01T00:00:00Zhttps://www.mdpi.com/1660-4601/18/21/11368https://doaj.org/toc/1661-7827https://doaj.org/toc/1660-4601In the last decade, the incidence and severity of <i>Clostridioides difficile</i> infections (CDIs) in humans have been increasing and community-associated infections have been described. For these reasons, the interest in <i>C. difficile</i> in food and in food animals has increased, suggesting other possible sources of <i>C. difficile</i> acquisition. This study evaluated the presence of <i>C. difficile</i> on pig carcasses at the slaughterhouse and in pork products in Central Italy. The contamination rate on pig carcasses was 4/179 (2.3%). Regarding food samples, a total of 216 pork products were tested (74 raw meat preparations and 142 ready-to-eat food samples made by cured raw meat). The real-time PCR screening was positive for 1/74 raw meat preparation (1.35%) and for 1/142 ready-to-eat food samples (0.7%) <i>C. difficile</i> was isolated only from the raw meat preparation (pork sausage). All the isolated strains were toxigenic and susceptible to all the tested antibiotics. Strains isolated from carcass samples displayed A+B+CDTa+CDTb+ profile, were toxinotype IV and belonged to the same ribotype arbitrary named TV93, while the one isolated from food samples displayed A+B+CDTa-CDTb- profile and it was not possible to determine ribotype and toxinotype, because it was lost after freeze storage. It was concluded that the prevalence of <i>C. difficile</i> in the pork supply chain is very low.Caterina LicciardiSara PrimavillaRossana RoilaAlessia LupattelliSilvana FarnetiGiuliana BlasiAnnalisa PetruzzelliIlenia DrigoEnrico Di Raimo MarrocchiMDPI AGarticle<i>Clostridioides difficile</i>pig carcassespork productsfood safetyreal-time PCRribotypeMedicineRENInternational Journal of Environmental Research and Public Health, Vol 18, Iss 11368, p 11368 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Clostridioides difficile</i> pig carcasses pork products food safety real-time PCR ribotype Medicine R
spellingShingle	<i>Clostridioides difficile</i> pig carcasses pork products food safety real-time PCR ribotype Medicine R Caterina Licciardi Sara Primavilla Rossana Roila Alessia Lupattelli Silvana Farneti Giuliana Blasi Annalisa Petruzzelli Ilenia Drigo Enrico Di Raimo Marrocchi Prevalence, Molecular Characterization and Antimicrobial Susceptibility of <i>Clostridioides difficile</i> Isolated from Pig Carcasses and Pork Products in Central Italy
description	In the last decade, the incidence and severity of <i>Clostridioides difficile</i> infections (CDIs) in humans have been increasing and community-associated infections have been described. For these reasons, the interest in <i>C. difficile</i> in food and in food animals has increased, suggesting other possible sources of <i>C. difficile</i> acquisition. This study evaluated the presence of <i>C. difficile</i> on pig carcasses at the slaughterhouse and in pork products in Central Italy. The contamination rate on pig carcasses was 4/179 (2.3%). Regarding food samples, a total of 216 pork products were tested (74 raw meat preparations and 142 ready-to-eat food samples made by cured raw meat). The real-time PCR screening was positive for 1/74 raw meat preparation (1.35%) and for 1/142 ready-to-eat food samples (0.7%) <i>C. difficile</i> was isolated only from the raw meat preparation (pork sausage). All the isolated strains were toxigenic and susceptible to all the tested antibiotics. Strains isolated from carcass samples displayed A+B+CDTa+CDTb+ profile, were toxinotype IV and belonged to the same ribotype arbitrary named TV93, while the one isolated from food samples displayed A+B+CDTa-CDTb- profile and it was not possible to determine ribotype and toxinotype, because it was lost after freeze storage. It was concluded that the prevalence of <i>C. difficile</i> in the pork supply chain is very low.
format	article
author	Caterina Licciardi Sara Primavilla Rossana Roila Alessia Lupattelli Silvana Farneti Giuliana Blasi Annalisa Petruzzelli Ilenia Drigo Enrico Di Raimo Marrocchi
author_facet	Caterina Licciardi Sara Primavilla Rossana Roila Alessia Lupattelli Silvana Farneti Giuliana Blasi Annalisa Petruzzelli Ilenia Drigo Enrico Di Raimo Marrocchi
author_sort	Caterina Licciardi
title	Prevalence, Molecular Characterization and Antimicrobial Susceptibility of <i>Clostridioides difficile</i> Isolated from Pig Carcasses and Pork Products in Central Italy
title_short	Prevalence, Molecular Characterization and Antimicrobial Susceptibility of <i>Clostridioides difficile</i> Isolated from Pig Carcasses and Pork Products in Central Italy
title_full	Prevalence, Molecular Characterization and Antimicrobial Susceptibility of <i>Clostridioides difficile</i> Isolated from Pig Carcasses and Pork Products in Central Italy
title_fullStr	Prevalence, Molecular Characterization and Antimicrobial Susceptibility of <i>Clostridioides difficile</i> Isolated from Pig Carcasses and Pork Products in Central Italy
title_full_unstemmed	Prevalence, Molecular Characterization and Antimicrobial Susceptibility of <i>Clostridioides difficile</i> Isolated from Pig Carcasses and Pork Products in Central Italy
title_sort	prevalence, molecular characterization and antimicrobial susceptibility of <i>clostridioides difficile</i> isolated from pig carcasses and pork products in central italy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f8d28f1f3cb8478d9a65f1c37f93f492
work_keys_str_mv	AT caterinalicciardi prevalencemolecularcharacterizationandantimicrobialsusceptibilityoficlostridioidesdifficileiisolatedfrompigcarcassesandporkproductsincentralitaly AT saraprimavilla prevalencemolecularcharacterizationandantimicrobialsusceptibilityoficlostridioidesdifficileiisolatedfrompigcarcassesandporkproductsincentralitaly AT rossanaroila prevalencemolecularcharacterizationandantimicrobialsusceptibilityoficlostridioidesdifficileiisolatedfrompigcarcassesandporkproductsincentralitaly AT alessialupattelli prevalencemolecularcharacterizationandantimicrobialsusceptibilityoficlostridioidesdifficileiisolatedfrompigcarcassesandporkproductsincentralitaly AT silvanafarneti prevalencemolecularcharacterizationandantimicrobialsusceptibilityoficlostridioidesdifficileiisolatedfrompigcarcassesandporkproductsincentralitaly AT giulianablasi prevalencemolecularcharacterizationandantimicrobialsusceptibilityoficlostridioidesdifficileiisolatedfrompigcarcassesandporkproductsincentralitaly AT annalisapetruzzelli prevalencemolecularcharacterizationandantimicrobialsusceptibilityoficlostridioidesdifficileiisolatedfrompigcarcassesandporkproductsincentralitaly AT ileniadrigo prevalencemolecularcharacterizationandantimicrobialsusceptibilityoficlostridioidesdifficileiisolatedfrompigcarcassesandporkproductsincentralitaly AT enricodiraimomarrocchi prevalencemolecularcharacterizationandantimicrobialsusceptibilityoficlostridioidesdifficileiisolatedfrompigcarcassesandporkproductsincentralitaly
_version_	1718432332567805952

Prevalence, Molecular Characterization and Antimicrobial Susceptibility of <i>Clostridioides difficile</i> Isolated from Pig Carcasses and Pork Products in Central Italy

Ejemplares similares