Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules

por Arik Shams, Sean A. Higgins, Christof Fellmann, Thomas G. Laughlin, Benjamin L. Oakes, Rachel Lew, Shin Kim, Maria Lukarska, Madeline Arnold, Brett T. Staahl, Jennifer A. Doudna, David F. Savage
Publicado 2021

An improved biolistic delivery and analysis method for evaluation of DNA and CRISPR-Cas delivery efficacy in plant tissue

por Kyle Miller, Alan L. Eggenberger, Keunsub Lee, Fei Liu, Minjeong Kang, Madison Drent, Andrew Ruba, Tyler Kirscht, Kan Wang, Shan Jiang
Publicado 2021

Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform

por Christian Munck, Ravi U. Sheth, Daniel E. Freedberg, Harris H. Wang
Publicado 2020

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

por Zhen Liang, Kunling Chen, Tingdong Li, Yi Zhang, Yanpeng Wang, Qian Zhao, Jinxing Liu, Huawei Zhang, Cuimin Liu, Yidong Ran, Caixia Gao
Publicado 2017

Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment

por Seung-Hun Kang, Wi-jae Lee, Ju-Hyun An, Jong-Hee Lee, Young-Hyun Kim, Hanseop Kim, Yeounsun Oh, Young-Ho Park, Yeung Bae Jin, Bong-Hyun Jun, Junho K. Hur, Sun-Uk Kim, Seung Hwan Lee
Publicado 2020

Functional linkage of gene fusions to cancer cell fitness assessed by pharmacological and CRISPR-Cas9 screening

por Gabriele Picco, Elisabeth D. Chen, Luz Garcia Alonso, Fiona M. Behan, Emanuel Gonçalves, Graham Bignell, Angela Matchan, Beiyuan Fu, Ruby Banerjee, Elizabeth Anderson, Adam Butler, Cyril H. Benes, Ultan McDermott, David Dow, Francesco Iorio, Euan Stronach, Fengtang Yang, Kosuke Yusa, Julio Saez-Rodriguez, Mathew J. Garnett
Publicado 2019

Whole chromosome loss and genomic instability in mouse embryos after CRISPR-Cas9 genome editing

por Stamatis Papathanasiou, Styliani Markoulaki, Logan J. Blaine, Mitchell L. Leibowitz, Cheng-Zhong Zhang, Rudolf Jaenisch, David Pellman
Publicado 2021

In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering

por Qianxin Wu, Quentin R. V. Ferry, Toni A. Baeumler, Yale S. Michaels, Dimitrios M. Vitsios, Omer Habib, Roland Arnold, Xiaowei Jiang, Stefano Maio, Bruno R. Steinkraus, Marta Tapia, Paolo Piazza, Ni Xu, Georg A. Holländer, Thomas A. Milne, Jin-Soo Kim, Anton J. Enright, Andrew R. Bassett, Tudor A. Fulga
Publicado 2017

Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets

por Joshua M. Dempster, Clare Pacini, Sasha Pantel, Fiona M. Behan, Thomas Green, John Krill-Burger, Charlotte M. Beaver, Scott T. Younger, Victor Zhivich, Hanna Najgebauer, Felicity Allen, Emanuel Gonçalves, Rebecca Shepherd, John G. Doench, Kosuke Yusa, Francisca Vazquez, Leopold Parts, Jesse S. Boehm, Todd R. Golub, William C. Hahn, David E. Root, Mathew J. Garnett, Aviad Tsherniak, Francesco Iorio
Publicado 2019

CRISPR/Cas9 editing of APP C-terminus attenuates β-cleavage and promotes α-cleavage

por Jichao Sun, Jared Carlson-Stevermer, Utpal Das, Minjie Shen, Marion Delenclos, Amanda M. Snead, So Yeon Koo, Lina Wang, Dianhua Qiao, Jonathan Loi, Andrew J. Petersen, Michael Stockton, Anita Bhattacharyya, Mathew V. Jones, Xinyu Zhao, Pamela J. McLean, Andrew A. Sproul, Krishanu Saha, Subhojit Roy
Publicado 2019

Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping

por Peter Gee, Mandy S. Y. Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F. Yang, Mio Iwasaki, Xiou H. Wang, Matthew A. Waller, Nanako Shirai, Yasuko O. Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita, Kumiko A. Iwabuchi, Masahiko Yasuda, Huaigeng Xu, Takeshi Noda, Jun Komano, Hidetoshi Sakurai, Naoto Inukai, Akitsu Hotta
Publicado 2020

CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

por Toon Swings, David C. Marciano, Benu Atri, Rachel E. Bosserman, Chen Wang, Marlies Leysen, Camille Bonte, Thomas Schalck, Ian Furey, Bram Van den Bergh, Natalie Verstraeten, Peter J. Christie, Christophe Herman, Olivier Lichtarge, Jan Michiels
Publicado 2018

Improved CRISPR genome editing using small highly active and specific engineered RNA-guided nucleases

por Moritz J. Schmidt, Ashish Gupta, Christien Bednarski, Stefanie Gehrig-Giannini, Florian Richter, Christian Pitzler, Michael Gamalinda, Christina Galonska, Ryo Takeuchi, Kui Wang, Caroline Reiss, Kerstin Dehne, Michael J. Lukason, Akiko Noma, Cindy Park-Windhol, Mariacarmela Allocca, Albena Kantardzhieva, Shailendra Sane, Karolina Kosakowska, Brian Cafferty, Jan Tebbe, Sarah J. Spencer, Scott Munzer, Christopher J. Cheng, Abraham Scaria, Andrew M. Scharenberg, André Cohnen, Wayne M. Coco
Publicado 2021

Small molecules enhance CRISPR/Cas9-mediated homology-directed genome editing in primary cells

por Guoling Li, Xianwei Zhang, Cuili Zhong, Jianxin Mo, Rong Quan, Jie Yang, Dewu Liu, Zicong Li, Huaqiang Yang, Zhenfang Wu
Publicado 2017

Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes

por Jasper Edgar Neggers, Bert Kwanten, Tim Dierckx, Hiroki Noguchi, Arnout Voet, Lotte Bral, Kristien Minner, Bob Massant, Nicolas Kint, Michel Delforge, Thomas Vercruysse, Erkan Baloglu, William Senapedis, Maarten Jacquemyn, Dirk Daelemans
Publicado 2018

In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing

por Yu Wang, Haijiao Cheng, Yang Liu, Ye Liu, Xiao Wen, Kun Zhang, Xiaomeng Ni, Ning Gao, Liwen Fan, Zhihui Zhang, Jiao Liu, Jiuzhou Chen, Lixian Wang, Yanmei Guo, Ping Zheng, Meng Wang, Jibin Sun, Yanhe Ma
Publicado 2021

Locus-Specific DNA Methylation Editing in Melanoma Cell Lines Using a CRISPR-Based System

por Jim Smith, Rakesh Banerjee, Reema Waly, Arthur Urbano, Gregory Gimenez, Robert Day, Michael R. Eccles, Robert J. Weeks, Aniruddha Chatterjee
Publicado 2021

Systematic Studies of the Circadian Clock Genes Impact on Temperature Compensation and Cell Proliferation Using CRISPR Tools

por Yue Wu, Tian Tian, Yin Wu, Yu Yang, Yunfei Zhang, Ximing Qin
Publicado 2021

CRISPR Screen Reveals that EHEC’s T3SS and Shiga Toxin Rely on Shared Host Factors for Infection

por Alline R. Pacheco, Jacob E. Lazarus, Brandon Sit, Stefanie Schmieder, Wayne I. Lencer, Carlos J. Blondel, John G. Doench, Brigid M. Davis, Matthew K. Waldor
Publicado 2018

<italic toggle="yes">In Vitro</italic> CRISPR/Cas9 System for Efficient Targeted DNA Editing

por Yunkun Liu, Weixin Tao, Shishi Wen, Zhengyuan Li, Anna Yang, Zixin Deng, Yuhui Sun
Publicado 2015